1-溴丙烷亚急性吸入染毒对大鼠大脑NR2B和GluR2影响  

Effects of 1-bromopropane sub-acute inhalation on NR2B and GluR2 in the brain of rats

作　　者：张伟[1] 黄建勋[2] 王海兰[2] 蒋柳权[1] 戎伟丰[2] 宋向荣[2] 

机构地区：[1]山西医科大学公共卫生学院,山西太原030001 [2]广东省职业病防治院,广东省职业病防治重点实验室,广东广州510300

出　　处：《中国职业医学》2013年第4期301-304,共4页China Occupational Medicine

基　　金：国家自然科学基金资助项目(81202246,30972459);广东省职业病防治重点实验室(2012A061400007)

摘　　要：目的探讨1-溴丙烷(1-BP)对大鼠大脑离子型谷氨酸受体NR2B亚基和GluR2亚基的影响。方法 无特定病原体级成年雄性Wistar大鼠36只,随机分为对照组、低剂量组、中剂量组和高剂量组4组,于动式吸入染毒柜内分别暴露于质量浓度为0、1 250、2 500和5 000 mg/m3的1-BP气体,8 h/d,每周染毒5 d,连续4周。实验结束后处死大鼠,分离脑组织,应用蛋白质印迹法和实时荧光定量聚合酶链式反应法测定NR2B和GluR2的蛋白和基因表达水平。结果 中、高剂量组大鼠大脑NR2B和GluR2的蛋白表达水平均高于对照组和低剂量组(P<0.05或P<0.01)。与对照组相比,高剂量组大鼠大脑NR2B mRNA表达水平增加(P<0.05),各剂量组大鼠大脑GluR2 mRNA表达水平均增加(P<0.05或P<0.01)。结论 一定水平的1-BP亚急性吸入染毒能增加大鼠大脑NR2B和GluR2的蛋白表达。Objective To explore the effects of 1-bromopropane （1-BP） on inotropic glutamate receptor subunits NR2B and GluR2 in the brain of rats. Methods Thirty-six male adult specific pathogen-free Wistar rats were randomly divided into 4 groups （control, low-dose, medium-dose and high-dose） and then respectively exposed to 1-BP vapor for 4 weeks （8 h/day, 5 days/week） at the concentrations of 0, 1 250, 2 500 and 5 000 mg/m3 in a dynamic inhalation chamber system. Rats were sac- rificed at the end of the sub-acute exposure, and brains were removed for the following tests. The protein and mRNA expressions of NR2B and GluR2 in rats＇ brain were respectively detected by western blot and real-time fluorescent quantitative polymerase chain reaction. Results The protein expressions of brain＇s NR2B and GluR2 in the medium-dose group and the high-dose group were higher than those in the control group and the low-dose group （ P 〈 0.05 or P 〈 0. 01 ）. Compared with that of the control group, mRNA expressions of NR2B in rats＇ brain increased in the high-dose group （ P 〈 0. 05 ）, mRNA expressions of GluR2 in rats＇ brain increased in each of 1 -BP exposure groups （ P 〈 0.05 or P 〈 0. 01 ）. Conclusion The protein expressions of NR2B and GluR2 in rats＇ brain increased after sub-acute inhalation of 1-BP at certain concentrations.

关 键 词：1-溴丙烷 NR2B GLUR2 大鼠 

分 类 号：R135[医药卫生—劳动卫生]