姜黄素对转化生长因子-β1诱导大鼠肾小球系膜细胞增殖及Smads信号通路的影响  

Effect of glomerular mesangial cell proliferation and Smads signal pathway in the TGF-β1 induced rats

作　　者：李彧[1] 李亚东[1] 华颖[1] 田芃[1] 赵福建[1] 牛建昭[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国中医基础医学杂志》2013年第8期902-903,906,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81173642);教育部国家外专局学科创新引智基地项目(B07007)

摘　　要：目的:观察姜黄素对转化生长因子β1(TGF-β1)诱导的大鼠肾小球系膜细胞增殖及TGF-β1/Smads信号通路的影响。方法:采用TGF-β1诱导肾小球系膜细胞增殖和分泌胶原,实验分为空白对照组、单纯TGF-β1刺激组、姜黄素组3组。MTT法检测药物对肾小球系膜细胞增殖的抑制率,ELISA法检测肾小球系膜细胞Ⅰ型胶原的表达,Western blot法检测肾小球系膜细胞pSmad2的表达。结果:姜黄素能够显著抑制TGF-β1诱导的肾小球系膜细胞增殖(P<0.01)及Ⅰ型胶原分泌,并明显下调pSmad2蛋白表达。结论:姜黄素能部分阻断TGF-β/Smads信号传导通路的激活,抑制Ⅰ型胶原分泌及肾小球系膜细胞增殖。Objective ：To observe the effect of curcumin on TGF-β1 （transforming growth facto -β1, TGF-β1 ）induced proliferation and Smads pathway in rat GMC （glomerular mesangial cell, GMC）. Methods：The experiment was divided into 3 groups：blank,TGFβ1 control,curcumin groups. MTT method was adopted to examine the proliferation and inhibition rate of GMC. Enzyme-linked immunosorbent assay was used to determine the production of type I collagen in cultured GMC. The production of pSmad2 in GMC was detected by western blot. Results： Curcumin significantly inhibited the proliferation of GMC（P 〈 0. 01 ） and also inhibited the production of type I collagen, downregulated the expression pSmad2. Conclusion： Curcumin can partially block the activation o f pSmad signaling pathway in GMC, inhibit the excretion of collagen I and proliferation of GMC.

关 键 词：姜黄素 肾小球系膜细胞 转化生长因子-Β1 Ⅰ型胶原 

分 类 号：R285.5[医药卫生—中药学]