氟性骨损伤患者血清中骨形成抑制蛋白DKK-1的表达  

Expression of Serum DKK-1 Acted as Inhibitor of Bone Formation in Patients with Fluoride Bone Injury

作　　者：俞兵 刘晓利[2] 刘克俭[2] 徐建[2] 王文朋[2] 何晓宏 张裕曾[2] 

机构地区：[1]恩施州疾病预防控制中心,湖北445000 [2]华中科技大学同济医学院公共卫生学院,湖北430030

出　　处：《环境与职业医学》2013年第8期573-575,586,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助项目(编号:81072255)

摘　　要：[目的]观察氟性骨损伤患者血清dickkopf相关蛋白1(DKK-1)水平,分析氟性骨损伤的发生与DKK-1表达的相关性。[方法]采用自行设计的"氟与健康"量表对氟暴露人群进行问卷调查,在调查对象知情同意的前提下,采集静脉血及随机中段尿用于检测血氟、尿氟及血清中DKK-1蛋白浓度,并对其进行氟斑牙筛检及前臂正位X线检查。根据血氟浓度、氟斑牙及氟性骨损伤程度将调查对象分别分为不同氟负荷组、氟斑牙与否组及不同程度骨损伤组,分析不同组别之间DKK-1的差异。[结果]血清DKK-1在低、中、高氟负荷组的浓度分别为(19.60±0.52)、(17.74±0.80)、(14.31±1.20)μg/L,随氟负荷的增高血清DKK-1浓度依次降低,差异有统计学意义(P<0.05)。无氟斑牙组与氟斑牙组人群血清DKK-1水平差异无统计学意义(P>0.05)。无、轻、中、重度氟性骨损伤组血清DKK-1浓度分别为(19.16±0.76)、(15.86±1.35)、(13.27±0.45)、(12.42±0.04)μg/L;骨损伤组血清DKK-1浓度均低于无骨损伤组,差异有统计学意义(P<0.05)。[结论]氟性骨损伤患者血清DKK-1表达水平较低,提示高氟暴露可以致机体DKK-1蛋白表达下调,导致氟性骨损伤的发生。[ Objective ] To observe the expression of dickkopf-1 （DKK-1） in serum of fluoride bone injury （FBI） patients and analyze the relationship between fluoride bone injury occurrence and DKK-1 expression levels. [ Methods ] General information of fluoride exposed population was collected by a self-designed questionnaire. Venous blood and random midstream urine were sampled from the subjects to detect their serum and urinary fluoride concentrations and serum DKK-1 protein levels followed by informed consent. Dental fluorosis inspection and orthophoric forearm radiography were also performed. Differences in DKK-1 expressions were analyzed among participants grouped by serum fluoride level, dental fluorosis, and skeleton fluorosis diagnostic criteria. [ Results ] The concentrations of DKK-1 in the low, middle, and high fluoride burden groups were （9.60 ±0.52）, （17.74 ± 0.80）, and （14.31 ± 1.20） μg/L, respectively, and the difference was statistically significant among the three fluoride burden groups （P〈 0.05）. There was no significant difference in DKK-1 expressions between the dental fluorosis group and the no dental fluorosis group （P〉 0.05）. The levels of DKK-1 in the no, mild, moderate, and heavy FBI groups were （19.16 ±0.76）, （15.86 ±1.35）, （13.27 ± 0.45）, and （12.42 ± 0.04） μg/L, respectively, with statistical differences （P〈0.05）. [ Conclusion ] The level of DKK-1 is significantly decreased in patients with FBI. These results also indicate that long-term exposure to high level of fluoride can down- regulate DKK-1 expression and might induce occurrence of FBI.

关 键 词：氟性骨损伤 血氟 尿氟 dickkopf相关蛋白1 

分 类 号：R131[医药卫生—劳动卫生]