白藜芦醇对高脂饮食去卵巢肥胖大鼠脑组织炎症反应及海马Aβ_(1-42)水平的影响  

Effects of resveratrol on brain inflammation and the level of Aβ_(1-42) in hippocampus from ovariectomized obese rats fed with a high-fat diet

作　　者：苗玉连[1] 武传龙[1] 刘金波[1] 张晓黎[1] 

机构地区：[1]山东大学齐鲁医院内分泌科,济南250012

出　　处：《山东大学学报（医学版）》2013年第8期17-21,共5页Journal of Shandong University:Health Sciences

基　　金：山东省优秀中青年科学家科研奖励基金(BS2009YY038);山东大学自主创新基金(2012TS162)

摘　　要：目的探讨白藜芦醇对高脂饮食去卵巢肥胖大鼠海马组织炎症反应及β淀粉样蛋白1-42(Aβ1-42)水平的影响。方法健康3月龄Wistar雌性大鼠30只随机分为假手术普通饮食组(SHAM组)、去卵巢高脂饮食组(OH组)、白藜芦醇[40 mg/(kg·d)-1]干预组(OHR组)。相应干预3个月后检测大鼠血清雌二醇(E2)、胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平,RT-PCR法检测海马组织中白细胞介素6(IL-6)及肿瘤坏死因子α(TNF-α)mRNA的表达,ELISA法检测海马匀浆IL-6、TNF-α与β淀粉样蛋白1-42(Aβ1-42)表达。结果 OH组大鼠血清E2水平较SHAM组明显降低(P<0.01),OHR组大鼠血清中E2水平较OH组显著升高(P<0.05);OH组大鼠血清TC、LDL-C含量较SHAM组升高(P<0.01),OHR组大鼠血清TC含量较OH组降低(P<0.01);OH组大鼠海马组织IL-6、TNF-αmRNA及IL-6、TNF-α、Aβ1-42蛋白含量较SHAM组明显升高(P<0.01),OHR组IL-6、TNF-αmRNA及Aβ1-42蛋白含量较OH组明显降低(P<0.05)。结论白藜芦醇能减轻高脂饮食去卵巢肥胖大鼠海马组织中Aβ1-42的沉积,其机制可能与白藜芦醇升高大鼠血清E2水平、降低血清TC含量、改善肥胖相关大脑炎症反应有关。Objective To explore the effects of resveratrol on the neuroinflammation and the level of Aβ1-42 in hippo- campus from ovariectomized obese rats fed with a high-fat diet. Methods Thirty female Wistar rats aged 3 months were randomly divided into three groups： the sham operation fed with low-fat diet （SHAM） group; the ovariectomy fed with high-fat diet （OH） group ; the ovariectomy plus high-fat diet treated with resveratrol [ 40 mg/（ kg· d） -1 ] （OHR） group. After three months of administration, the rats blood was collected from arteria cruralis to detect the serum concentration of total TC, TG, LDL-C, HDL-C and E2 ; the expression of IL-6 and TNF-ct mRNA in hippocampus was determined by real-time PCR; the levels of IL-6, TNF-α and Aβ1-42 protein in hippocampal tissues were observed by ELISA. Results The level of serum E2 was significantly lower in the OH group than that in the SHAM group （ P 〈 0.01 ）. However, rats in the OHR group had a higher level of serum E2 than rats in the OH group （ P 〈 0.05 ） ; The levels of serum TC and LDL-C in the OH group were higher than those in the SHAM group （ P 〈 0.01 ）, and rats in the OHR group had a lower level of serum TC（ P 〈 0.01 ） ; Expression of IL-6, TNF-α mRNA and IL-6, TNF-ot, Aβ1-42 proteins in the OH group significantly increased compared with that in the SHAM group （P 〈 0.01 ）. Nevertheless, rats in the OHR group showed significantly lower expression of IL-6, TNF-α mRNA and IL-6, TNF-α, Aβ1-42 proteins ratsin the OH group （ P 〈 0.05 ）. Conclusion Resveratrol can attenuate the accumulation of Aβ1-42 in hippocampus from ovariectomized obese rats fed with a high-fat diet. The mechanism may be associated with its increasing the level of E2, decreasing the content of TC and ameliorating the obesity-related brain inflammatory responses.

关 键 词：白藜芦醇 肥胖 海马 炎症反应 AΒ1-42 

分 类 号：R589[医药卫生—内分泌] R285[医药卫生—内科学]