一种高效的蚕豆染色体加倍方法探讨  

An Efficient Method for Chromosome Doubling of Horsebean

作　　者：王晓雯[1] 桑贤春[1] 

机构地区：[1]西南大学农学与生物科技学院,南方山地农业教育部工程研究中心,重庆400716

出　　处：《河南农业科学》2013年第6期42-45,共4页Journal of Henan Agricultural Sciences

基　　金：中央高校基本科研业务费项目(XDJK2011c016);西南大学本科生试验教学改革项目

摘　　要：为解决传统植物染色体加倍方法效率低、费工费时、中期分裂相细胞少等问题,采用4种质量浓度秋水仙素对蚕豆侧根进行处理,检测加倍效果。结果表明,以0.5～1.0cm长的蚕豆侧根为材料,用0.10mg/g的秋水仙素处理48h时,根尖的膨大率最高,为92.94%,膨大根尖中部至根冠区的细胞诱导率为28.20%,显著高于中部至伸长区。侧根平均8条,与传统的主根加倍方法相比,工作效率提高了8倍,且显微镜下观测到的中期分裂相细胞更多。因此,该方法不仅适合植物染色体加倍试验的教学,也为其他植物的染色体加倍提供了借鉴。To overcome the defects of inefficiency,labor consuming and lower induction rate in traditional chromosome doubling techniques,the lateral roots of horsebean were treated by 4 concentrations of colchicine and the doubling efficiency was tested.The results demonstrated that when the 0.5-1.0 cm lateral roots were used as material and treated for 48 h with 0.10 mg/g colchicine,the expanded rate of root tips reached to 92.94%.The cell induction rate of expanded root was 28.20% in the region from middle to root cap,which was significantly higher than in the region from middle to elongation zone.The present method displayed a higher doubling efficiency,increasing by 8 times compared with the traditional method（treating the taproot）,and more metaphase cells were observed by means of microscope.Therefore,the method not only suits for teaching in plant chromosome doubling,but also offers a reference for chromosome doubling of other plants.

关 键 词：秋水仙素 蚕豆 侧根 染色体加倍 

分 类 号：Q3-3[生物学—遗传学]