盐芥愈伤组织的诱导及悬浮细胞的培养特性  

Callus Induction and Culture Characteristics of Suspension Cells of Thellungiella halophila

作　　者：安会灵[1] 徐小静[1] 张瑾[1] 韦善君[1] 周宜君[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《河南农业科学》2013年第6期46-50,共5页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31100287);中央高校基本科研业务费专项资金项目(1112KYZY43);中央民族大学"985"项目(MUC98504-14;MUC98507-08)

摘　　要：为建立稳定的盐芥悬浮细胞系,以盐芥(山东生态型)为材料,研究了不同激素及其不同质量浓度配比下叶柄及叶片愈伤组织的诱导情况,并初步探讨了盐芥悬浮细胞的培养特性。结果表明:6-BA与2,4-D、NAA不同质量浓度组合均能诱导出愈伤,其中叶柄诱导率为100%,叶片诱导率偏低且出愈时间晚于叶柄。并且6 BA与NAA组下2种外植体均能分化出芽,2.0 mg/L6 BA+0.1mg/L NAA组合下盐芥叶柄的分化率高达57%。按照通常每7d继代1次,50mL液体培养基中盐芥悬浮细胞接种量以10.0mL比较合适。In order to establish a stable suspension cell line of Thellungiela halophila,petioles and leaves from T.halophila（Shandong ecotype） were used as explants to investigate the effect of different hormones and the concentration on callus induction,and then the effect of diluting ratio on the growth of suspension cells was discussed.The results showed that MS＋6-BA＋2,4-D and MS＋6-BA＋NAA both induced massive callus.The petioles had higher callus induction ratio than leaves,even up to 100%,and callus from leaves formed later than that from petioles.It was indicated that MS＋6-BA＋NAA could also help petioles and leaves to differentiate adventitious buds,with 57% petiole differentiation ratio.In the growth of suspension cells,it was found that theinoculation volume of 10.0 mL suspension cells into 50 mL liquid medium was advisable in thesubculture experiment once a week.

关 键 词：盐芥 叶片 叶柄 愈伤组织 悬浮细胞 

分 类 号：Q943.1[生物学—植物学]