番茄茎枯病病原菌鉴定  

Identification of Pathogen Causing Tomato Stem Blight

作　　者：裴冬丽[1] 洪权春[1] 丁锦平[1] 李成伟[1,2] 

机构地区：[1]商丘师范学院生命科学学院,植物与微生物互作重点实验室,河南商丘476000 [2]周口师范学院生命科学学院,植物遗传与分子育种重点实验室,河南周口466001

出　　处：《河南农业科学》2013年第6期95-97,共3页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31071807)

摘　　要：番茄茎枯病主要危害番茄的茎和果实,严重影响番茄的产量和品质,为此,采用组织分离法对河南商丘地区番茄茎枯病病原菌进行分离、纯化,测定其致病性,并进行形态学和分子生物学鉴定。结果表明,番茄茎枯病病原菌为链格孢菌(Alternaria alternate),其rDNA ITS序列与Gen-Bank中苹果链格孢菌(A.alternate)的ITS序列同源性为100%,与链格孢属的其他小孢子种聚在一个大的分支上。番茄茎枯病病原菌的鉴定为该病害的防治奠定了基础。Tomato stem blight mainly causes stems and fruits hurt,resulting in reduced yield and quality.The pathogen of this disease was isolated by tissue isolation way,and further pathogenicity test,morphological and molecular identification were done.The results showed that the pathogen was Alternaria alternate,whose rDNA ITS sequence shared a 100% identity with the ITS sequence of Alternaria alternate from apple,and was clustered in one branch with other small-spored species of Alternaria Nees.Identification of the pathogen of tomato stem blight will provide theoretical basis for control of the disease.

关 键 词：番茄茎枯病 病原鉴定 链格孢菌 ITS序列 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]