Morphological study of Schwann cells remyelination in contused spinal cord of rats  

作　　者：LI Yue ZHANG Lu ZHANG Jie-yuan LIU Zheng DUAN Zhao-xia LI Bing-cang 

机构地区：[1]Neurosurgery Department, 451 Hospital of ChinesePeople's Liberation Army, Xi'an 710054, China [2]Life School, Southwest University, Chongqing 400715,China [3]State Key Laboratory of Trauma, Burn and CombinedInjury, Research Institute of Surgery and Daping Hospital,Third Military Medical University, Chongqing 400042, China [4]Physiological Department, Chongqing MedicalUniversity, Chongqing 400042, China

出　　处：《Chinese Journal of Traumatology》2013年第4期225-229,共5页中华创伤杂志（英文版）

摘　　要：Objective： To study the role and effect of Schwann cells （SCs） remyelination in contused spinal cord. Methods： Green fluorescence protein expressing-SCs were transplanted into the epicenter, rostral and caudal tis- sues of the injury site at 1 week after the spinal cords were contused. At 6 weeks, the spinal cords were removed for cryosections, semithin sections and ultrathin sections, and then immunocytochemical staining of myelin basic protein （MBP）, P0 protein （P0） and S 100 protein （S 100） was carried out on the cryosections. Qualitative and semiquantitative analyses were performed on the cryosections and semithin sections. Ultrastructure ofmyelinated fibers was observed on the ultrathin sections under electron microscope. Results： Transplanted SCs and myelinated fibers im- munocytochemically labeled by MBP, PO as well as S 100 distributed in whole injured area. The quantity of myeli- nated fibers labeled by the three myelin proteins showed no statistical difference, however, which was significantly larger than that of controls. On the semithin sections, the experi- mental group demonstrated more myelinated fibers in the injured area than the controls, but the fibers had smaller diameter and thinner myelin sheath under electron microscope. Conclusion： SCs can promote regeneration of injured nerve fibers and enhance remyelination, which may be his- tological basis of SCs-mediated functional repair of injured spinal cords.

关 键 词：Spinal cord injury Schwann cells My- elin basic protein Myelin PO protein S100 proteins 

分 类 号：X[环境科学与工程]