Microarray partition using a recycled marker pen and neutral balsam  

作　　者：Li Cheng Shuiuan Guo Shengce Tao 

机构地区：[1]Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai J iao Tong University, Shanghai 200240, China [2]State Key Laboratory of Oncogenes and Related Genes, Shanghai 200240, China [3]School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

出　　处：《Acta Biochimica et Biophysica Sinica》2013年第8期706-708,共3页生物化学与生物物理学报（英文版）

摘　　要：Microarrays are very powerful tools for both basic research and clinical studies [1 ]. There are a variety of types of micro arrays, e.g. DNA microarrays, protein microarrays, carbohy drate arrays, tissue microarrays [2] etc. They have been widely applied for numerous applications from gene expres sion monitoring [3], single nucleotide polymorphism analysis [4] to protein protein interaction profiling [5], protein small molecule interaction analysis [6], etc. The most wellrecognized micmarrays usually carry thou sands to millions of probes on a single array. To save cost, time, and labor, it is a good practice to create subarrays on a single standard substrate slide in a layout compatible with the standard multichannel pipette, thus facilitating high throughput operation. As to effectively analyze multiple samples on a targeted microarray, tile subamlys must be physically partitioned to prevent crosscontamination. To effectively partition subarrays on a single slide, several types of incubation frames have been developed, e.g. FrameSealTM incubation chamber （BioRad, Hercules, USA）, SmartGridTM （CapitalBio Corporation, Beijing, China）, and Whatman： FAST slide incubation chambers （GE Healthcare, Bethesda, USA）. Usually, these frames are adhesive on one side. They could be fastened and stuck to the microarray and could be easily removed after incubation. However, these chambers are and layout of the chamber is not reusable and tile number fixed. If the chamber is not properly handled, leaking will occur and thus cause cross contamination. To increase the reusability of the chamber, nonadhesive chamber coupled with a clipping toolkit is also available, e.g. the ArraySlide chamber and ArraySlide gasket （Gel Company, San Francisco, USA）. Another avail able partition method is to imprint prepatterned wax using a mold by a device named Slidelmprinter （Gel Company）. However, this device and the accessories are expensive. Here, we described a simple method for hydrophobic par tition of flexible numMicroarrays are very powerful tools for both basic research and clinical studies [1 ]. There are a variety of types of micro arrays, e.g. DNA microarrays, protein microarrays, carbohy drate arrays, tissue microarrays [2] etc. They have been widely applied for numerous applications from gene expres sion monitoring [3], single nucleotide polymorphism analysis [4] to protein protein interaction profiling [5], protein small molecule interaction analysis [6], etc. The most wellrecognized micmarrays usually carry thou sands to millions of probes on a single array. To save cost, time, and labor, it is a good practice to create subarrays on a single standard substrate slide in a layout compatible with the standard multichannel pipette, thus facilitating high throughput operation. As to effectively analyze multiple samples on a targeted microarray, tile subamlys must be physically partitioned to prevent crosscontamination. To effectively partition subarrays on a single slide, several types of incubation frames have been developed, e.g. FrameSealTM incubation chamber （BioRad, Hercules, USA）, SmartGridTM （CapitalBio Corporation, Beijing, China）, and Whatman： FAST slide incubation chambers （GE Healthcare, Bethesda, USA）. Usually, these frames are adhesive on one side. They could be fastened and stuck to the microarray and could be easily removed after incubation. However, these chambers are and layout of the chamber is not reusable and tile number fixed. If the chamber is not properly handled, leaking will occur and thus cause cross contamination. To increase the reusability of the chamber, nonadhesive chamber coupled with a clipping toolkit is also available, e.g. the ArraySlide chamber and ArraySlide gasket （Gel Company, San Francisco, USA）. Another avail able partition method is to imprint prepatterned wax using a mold by a device named Slidelmprinter （Gel Company）. However, this device and the accessories are expensive. Here, we described a simple method for hydrophobic par tition of flexible num

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术] TG174.42[自动化与计算机技术—计算机科学与技术]