富血小板血浆对大鼠游离脂肪移植物存活的作用  

Effects of platelet rich plasma on free fat graft＇s survival in rats

作　　者：周阎施婧[1] 余晶[1] 吴毅平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院整形外科,武汉430030

出　　处：《中华医学美学美容杂志》2013年第4期295-298,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的探讨富血小板血浆（plateletrichplasma，PRP）对游离脂肪移植物存活的作用。方法采用自身配对设计，获取Wistar大鼠脂肪组织和PRP，于24只Wistar大鼠背部分别注射以下3组脂肪移植物：A组为空白对照组；B组为PRP未激活组；C组为PRP激活组。术后1、2、3个月各处死8只大鼠，分别获取3组移植物标本，进行形态学和组织学观察，标本行HE染色，于光镜下观察脂肪组织存活情况，并进行病理学分析。结果形态学结果显示，术后1个月C组移植物直径明显大于A组（P〈0．05），而移植物体积比较，3组间差异均无统计学意义（P〉0．05）。组织学结果显示，术后1个月，C组小血管密度明显高于A组（P〈0．05），术后3个月，C组炎性反应明显轻于A组和B组（P〈0．05），而其他病理观察指标：脂肪组织完整性、纤维化程度及囊腔空泡形成，在各时间段3组间差异均无统计学意义（P〉0．05）。结论在Wistar大鼠模型上PRP对游离脂肪移植物的存活无明显促进作用。Objective To investigate the role of platelet rich plasma （PRP） on free fat graft＇s survival in a rat model. Methods Twenty-four rats were used in the experiment. For each rat, three groups of free fat grafts were injected into the subcutaneous layer of dorsum. Group A was the control group with 0.5 ml fat and 0. 12 ml normal saline. Group B received 0.5 ml fat and 0. 1 ml PRP with 0.02 ml normal saline. Group C was given combination of 0.5 ml fat and 0.1 ml PRP which was acti- vated by 0.02 ml calcium chloride. Eight rats were euthanised using pentobarbital sodium each time, 1, 2 and 3 months after fat transplantation. Specimens were acquired and stained with haematoxyline- eosin, and then the histological changes examined using light microscopy. All the data were statistical- ly analysed using paired t test to determine if there were differences between the study groups. Results Compared the volume of the fat grafts at 1 month, there was no deference between the groups （P〉0; 05）. However, the diameter of group C was significantly higher than group A （P〈0.05）. On histological examination, larger vascular density was observed in group C compared with group A at 1 month （P〈0.05）. And reduced inflammatory reaction was also observed in group C at 3 months （P〈0.05）. While other histological parameters were not statistically significant （P〉0.05）. Conclu- sions PRP does not enhance the survival of fat graft in the Wistar rat model.

关 键 词：富血小板血浆 生长因子 脂肪移植 

分 类 号：R622.9[医药卫生—整形外科]