Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs  

作　　者：Qiwei Shan Yanpeng Wang Kunling Chen Zhen Liang Jun Li Yi Zhang Kang Zhang Jinxing Liu Daniel F. Voytas Xuelian Zheng Yong Zhang Caixia Gao 

机构地区：[1]State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2]Department of Genetics, Cell Biology & Development and Center for Genome Engineering, 321 Church Street SE, University of Minnesota, Minneapolis, MN 55455, USA [3]Department of Biotechnology, School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China

出　　处：《Molecular Plant》2013年第4期1365-1368,共4页分子植物（英文版）

摘　　要：Dear Editor, In the past few years, the use of sequence-specific nucle- ases for efficient targeted mutagenesis has provided plant biologists with a powerful new approach for understanding gene function and developing new traits. These nucleases create DNA double-strand breaks at chromosomal targeted sites that are primarily repaired by the non-homologous end joining （NHEJ） or homologous recombination （HR） pathways. NHEJ is o^en imprecise and can introduce mutations at tar- get sites resulting in the loss of gene function. In contrast, HR uses a homologous DNA template for repair and can be employed to create site-specific sequence modifications or targeted insertions （Moynahan and Jasin, 2010）.Dear Editor, In the past few years, the use of sequence-specific nucle- ases for efficient targeted mutagenesis has provided plant biologists with a powerful new approach for understanding gene function and developing new traits. These nucleases create DNA double-strand breaks at chromosomal targeted sites that are primarily repaired by the non-homologous end joining （NHEJ） or homologous recombination （HR） pathways. NHEJ is o^en imprecise and can introduce mutations at tar- get sites resulting in the loss of gene function. In contrast, HR uses a homologous DNA template for repair and can be employed to create site-specific sequence modifications or targeted insertions （Moynahan and Jasin, 2010）.

分 类 号：Q691[生物学—生物物理学] S188[农业科学—农业基础科学]