低频电刺激对大鼠比目鱼肌废用性肌萎缩的影响及调控机制研究  

Inhibition Effect and Regulatory Mechanism of Low Frequency Electrical Stimulation on Soleus Muscles Fibres in Rats

作　　者：杜长亮[1] 贡建伟[1] 

机构地区：[1]南京航空航天大学体育部,江苏南京210016

出　　处：《天津体育学院学报》2013年第3期198-201,共4页Journal of Tianjin University of Sport

基　　金：中国博士后基金52批面上项目(项目编号:2012M521066)

摘　　要：研究长期低频电刺激对大鼠比目鱼肌废用性肌萎缩的影响,并初步探讨其调控机制。方法:以大鼠比目鱼肌为研究对象,采用后肢制动法建立废用性肌萎缩模型,SDH酶法检测长期低频电刺激对大鼠比目鱼肌肌纤维的影响,Real Time-PCR方法观察肌球蛋白重链I、IIa、IIx基因表达变化,Western blot检测p-JNK、p-ERK1/2、p-p38激酶蛋白表达。结果:低频电刺激30日后,大鼠比目鱼肌废用性肌萎缩情况明显好转,废用性肌萎缩模型中II型肌纤维向I型肌纤维的转化趋势得以逆转;肌球蛋白重链I、IIa、IIx基因表达变化同样显示由II型向I型转化的趋势;MAPK通路相关蛋白p-JNK、p-ERK1/2、p-p38均被激活。结论:长期低频电刺激可有效改善废用性肌萎缩,此过程可能与MAPK通路激活相关。The aim of this paper is to study the effect of low frequency electrical stimulation on the soleus muscles fibers in rats and to have a preliminary discussion of the regulatory mechanism. The method treated the rat＇s musculi soleus as the objects. The study adopted everyday LF electrical stimulation on the model of muscular atrophy of disuse 30 days after which it can be observed that the effect of transformation of the long term LF electrical stimulation of soleus muscular fiber under the SDH enzymic method; using the Real-time PCR method to observe the changes of myosin heavy chain （MHC） I, Ⅱa, Ⅱx genetic expression; Western blotting assay examined MAPK kinase protein expression. The results showed that after 30 days of LF electrical stimulation （ES）, the muscular atrophy of disuse was clearly better as it had a good reversion of the rat＇s soleus muscles of model Ⅱ transforming to model I; JNK/ MAPK pathway related protein p-JNK, p-ERK1/2, p-p38 were all activated. The conclusion is that the long term LF electrical stimulation can improve the muscular atrophy of disuse effectively and the process may result from the activation of JNK/MAPK pathway.

关 键 词：废用性肌萎缩 比目鱼肌 低频电刺激 

分 类 号：G804.6[文化科学—运动人体科学]