浙江省2008-2011年啮齿动物中汉坦病毒的分离及鉴定  

Isolation and identification of Hantavirus carried by rodents in Zhejiang province, China from 2008 to 2011

作　　者：徐芳[1] 朱函坪[1] 姚苹苹[1] 胡丹[2] 张云[2] 谢荣辉[1] 杨章女[1] 钱磊[1] 朱进[2] 

机构地区：[1]浙江省疾病预防控制中心微生物所,浙江杭州310051 [2]南京军区军事医学研究所,江苏南京210002

出　　处：《中国媒介生物学及控制杂志》2013年第4期285-288,共4页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金(81171609);浙江省自然科学基金(Y205749);江苏省自然科学基金(BK2010537)~~

摘　　要：目的分析浙江省肾综合征出血热(HFRS)疫区鼠形动物携带汉坦病毒状况及其型别。方法应用直接免疫荧光试验和RTPCR方法检测汉坦病毒抗原和核酸,阳性者用长爪沙鼠和VeroE6细胞进行病毒分离,对分离株的M片段基因进行序列测定和分析。结果 2008-2011年在浙江省HFRS疫区捕获的鼠形动物中以黑线姬鼠为优势鼠种;从该鼠鼠肺中共分离到6株汉坦病毒,序列分析表明分离株均为汉滩型汉坦病毒,其中Z521、524、534为H7亚型,TT-27、T-43、R88可能为新亚型;6株分离株之间的基因同源性较高,并与以往浙江省分离株具有较高同源性。结论 2008-2011年浙江省HFRS疫区鼠间存在汉滩型汉坦病毒流行。Objective To analyze the prevalence and genotypes of Hantavirus （HV） among rodents in the epidemic areas of hemorrhagic fever with renal syndrome （HFRS） in Zhejiang province, China from 2008 to 2011. Methods Direct immunofluorescence assay and RT-PCR were used to detect the antigen and nucleic acid of HV. Mongolian gerbils and Vero-E6 cells were used to isolate viruses from the HV-positive rodents, and the M segments of HV isolates were subjected to sequence analysis. Results Apodemus agrarius was the dominant species of the rodents captured in the epidemic areas of HFRS in Zhejiang province from 2008 to 2011. Six strains of HV were isolated from the lungs of this rodent species. The sequence analysis showed that the six isolates belonged to Hantaan virus （HTNV）, wherein Z521, 524, and 534 were classified as H7 subtype, and TF-27, T-43, and R88 might be classified as a new subtype. The six HV isolates showed high gene homology between them and with the strains previously isolated in Zhejiang province. Conclusion HTNV was prevalent in rodent in the epidemic areas of HFRS in Zhejiang province from 2008 to 2011.

关 键 词：汉坦病毒 病毒分离 M基因 序列分析 啮齿动物 

分 类 号：R372[医药卫生—病原生物学]