I03A-00723菌株发酵产物95-3的分离纯化及其体外活性  

Isolation,purification and in vitro activities of the fermentation product 95-3 from I03A-00723 strain

作　　者：董国霞[1] 马宵[1] 张玉琴[2] 贺晓波[2] 余利岩[2] 田霖[1] 谭亚军[1] 侯启明[1] 张月琴[2] 

机构地区：[1]中国食品药品检定研究院卫生部生物技术产品检定方法及其标准化重点实验室,北京100050 [2]中国医学科学院北京协和医学院医药生物技术研究所,北京100050

出　　处：《中国新药杂志》2013年第16期1882-1885,共4页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2009ZX09301-003;2009ZX09302-004)

摘　　要：目的:研究游动放线菌I03A-00723发酵产物95-3的分离纯化与体外生物学活性。方法:发酵I03A-00723菌株,收集菌丝体,用丙酮浸泡、乙酸乙酯萃取,通过硅胶柱色谱、半制备HPLC进行纯化。采用微稀释法和高通量荧光检测法分别测定化合物95-3的抗菌活性和对PDF酶的抑制活性。结果:确定了化合物95-3的分离纯化条件,HPLC显示其纯度达100%。体外活性实验结果表明:化合物95-3对粪肠球菌、屎肠球菌的敏感菌株和万古霉素耐药菌株均有较好的抗菌活性,对耐万古霉素屎肠球菌ATCC700221的MIC达到了3μg.mL-1;化合物95-3对PDF酶有较好的抑制活性。结论:化合物95-3具有较好的抗VRE活性和PDF酶抑制活性,有深入研究价值。Objective： To investigate the isolation, purification and in vitro biological activities of the ac- tive component 95-3 extracted from fermentation broth of Actinoplanes sichuanensis sp. nov I03A-00723. Methods： The fermentation myeelia of strain I03A-00723 were soaked by acetone and extracted by acetic ether. Colunm chro- matography of silica gel and semi-prep HPLC was used for purification of compound 95-3. The antibacterial activi- ties and inhibitive activity on PDF were determined by micro dilution and high-throughput fluorescence detection, respectively. Results： The methods of isolation and purification of compound 95-3 were established. The purity of compound 95-3 reached 100%. In vitro activity experiment showed that compound 95-3 had good antibacterial activi- ties against sensitive strains and vancomycin-resistant strains of E.faecalis and E.faecium, the MIC was 3 μg mL-1 against vancomycin-resistant E.faecium ATCC700221, and it had good inhibitive activity on PDF. Conclusion： The compound 95-3 has good antibacterial activities against VRE and inhibitive activity on PDF. It deserves to be studied further.

关 键 词：I03A-00723菌株 发酵产物 化合物95—3 分离纯化 活性研究 

分 类 号：R915[医药卫生—微生物与生化药学]