经输血传播病毒感染恒河猴的嗜肝性  

Hepatotropism of nonenveloped DNA virus in rhesus monkey infected by transfusion-transmitted virus

作　　者：肖红[1] 祁学忠[1] 杨守昌[1] 骆抗先[1] 

机构地区：[1]第一军医大学南方医院感染内科,广州510515

出　　处：《中华肝脏病杂志》2000年第5期291-292,共2页Chinese Journal of Hepatology

摘　　要：目的了解经输血传播病毒（TTV）的嗜肝性。方法从5只实验感染病毒的恒河猴各组织中抽提DNA，分别用病毒双链探针或单链负义探针，与吸附在纤维膜上的组织DNA进行斑点杂交。结果双链探针与肝、脾、胃、小肠和大肠杂交阳性，表明各该组织中都有无包膜DNA病毒的存在。单链负义探针与各该组织杂交，仅肝和小肠为阳性，表示存在可能作为病毒复制中间体的正义单链DNA。结论肝和小肠可能是病毒的复制场所，故无包膜DNA病毒可能具嗜肝性。Objective To study wether the nonenveloped DNA virus transmitted via blood transfusion ishepatotropic. Methods Total DNA was extracted from tissues of 5 experimentally infected Rhesus monkeys. A dot hy-bridization was done with virus double DNA strand probe or single antisense strand probe. Results Both single- anddouble-strand probes were hybridized with DNA of the liver, spleen, stomach, small intestine and colon. The virus was con-formed present in most of all the organs when double-strand probe was used. The positive was noted only in the liver andsmall intestine when single-strand antisense probe was used, which showed that in liver and small investine might have rep-licative intermediates of the virus. Conclusion It suggests that nonenveloped DNA virus replicate in the liver and smallintestine, so it might be hepatotropic.[

关 键 词：恒河猴 嗜肝性 TTV感染 DNA 斑点杂交 

分 类 号：R457[医药卫生—治疗学]