IL-18及IL-18结合蛋白在再生障碍性贫血患者血清的表达及临床意义  

Expression and clinical significance of serum IL-18 and IL-18 binding protein in aplastic anemia patients

作　　者：熊勤[1] 

机构地区：[1]汉川市人民医院,湖北汉川431600

出　　处：《临床血液学杂志（输血与检验）》2013年第3期401-402,404,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:探讨白细胞介素18(IL-18)及IL-18结合蛋白(IL-18BP)在再生障碍性贫血(AA)患者血清的表达及临床意义。方法:采用ELISA法检测25例AA患者(AA组)和25名正常人(对照组)血清IL-18及IL-18BP水平,实时定量RT-PCR方法检测IL-18及IL-18BP的mRNA表达。结果:AA组血清IL-18水平为(363.5±150.5)pg/ml,较对照组(178.6±100.2)pg/ml明显升高(P<0.01),重型AA患者的IL-18水平(442.5±124.1)pg/ml较慢性AA患者的IL-18水平(319.2±158.3)pg/ml提高更加明显(P<0.05);AA组血清IL-18BP水平(1694.2±516.3)pg/ml较对照组(1082.6±468.7)pg/ml明显升高(P<0.05),IL-18/IL-18BP比值在AA组明显升高(P<0.05)。RT-PCR结果证实AA组IL-18和IL-18BP较对照组均明显升高,IL-18/IL-18BP比值升高。结论:IL-18及IL-18BP与AA的发生和发展及其免疫机制异常密切相关,IL-18/IL-18BP平衡对临床诊治具有重要意义。Objective：To investigate the expression and clinical significance of serum interleukin-18（IL-18） and IL-18 binding protein （IL-18BP） in aplastic anemia （AA）patients. Method：Serum IL-18 and IL-18BP levels of 25 cases of AA patients and 25 healthy people were detected by ELISA,and IL-18 and IL-18BP mRNA expression was detected by real-time quantitative RT-PCR. Result：Compared with the normal control group （178.6±100.2） pg/ml ,serum IL-18 levels of AA was（363.5±150.5） pg/ml,which was significantly higher （P0.01）.The level of IL-18 in patients with severe AA （442.5±124.1） pg/ml was obviously higher than that of chronic AA patients （319.2±158.3） pg/ml. serum IL-18BP of AA（1694.2±516.3） pg/ml was significantly higher than that of normal control group （1082.6±468.7） pg/ml （P0.05）.IL-18/IL-18BP ratio was significantly higher in AA patients. RT-PCR results confirmed that IL-18 and IL-18BP of AA patients were significantly increased when comparing with the normal control group,and IL-18/IL-18BP ratio increased. Conclusion：IL-18 and IL-18BP might have a close relation to the occurrence and development of AA as well as its immune mechanisms.IL-18/IL-18BP balance might be of important significance for clinical diagnosis and treatment.

关 键 词：白细胞介素18 白细胞介素18结合蛋白 贫血再生障碍性 

分 类 号：R556[医药卫生—血液循环系统疾病]