不同剂量rmhTNF对大鼠移植性肝癌的治疗作用  

Treatment Effects of Different Doses of rmhTNF on Rats with Transplanted Hepatoma

作　　者：夏风[1] 石小红[2] 

机构地区：[1]湖北省妇幼保健院,湖北武汉430070 [2]嘉鱼县人民医院

出　　处：《湖北科技学院学报（医学版）》2013年第3期188-189,共2页Journal of Hubei University of Science and Technology(Medical Sciences)

摘　　要：目的观察肌肉注射重组改构人肿瘤坏死因子(rmhTNF)对大鼠移植性肝肿瘤(Walker-256)生长的抑制作用。方法将24只Wistar大鼠采用移植法建立Walker-256肝癌模型,移植成功后第11d行MRI检查,测量肿瘤体积(V1),再随机分成3组,对照组肌注生理盐水0.1ml,肌注1组肌注rmhTNF 16.7万u,肌注2组肌注rmhTNF 33.3万u,均为1次/d,连续10d后停止注射。第14d再行磁共振检查以确定肿瘤体积(V2),对3组间肿瘤生长率(V2/V1)进行比较。结果与对照组比较,肌注组对大鼠Walker-256移植性肝癌的生长均有较明显的抑制作用,但无明显剂量依赖关系。结论肌肉注射rmhTNF对大鼠Walker-256移植性肝肿瘤生长具有显著的抑制作用。Objective To observe the inhibiting effect of rmhTNF on rat Walker-256 transplanted hepa- toma used by intramuscular injection. Methods The model of hepatoma model of Wistar rats was established by walker-256 tumor implantation. The rats were randomly divided into three groups. IM groups were treated with rmhTNF1.67 or 3.33 × 10^6 unit, respective, and the control group was injected intramuscularly with normal sa- line ,once a day. The tumor volume （V1 or V2） was measured by magnetic resonance imaging 11 days after im- plantation or14 days after injection. Results Compare with the control group, the growth of Walker-256 trans- planted hepatoma in the therapy group was significantly decreased without dose-dependence. Conclusion Intra- muscular injection of rmhTNF can significantly inhibit the growth of rat Walker-256 transplanted hepatoma.

关 键 词：Walker-256移植性肝肿瘤 肌肉注射 肿瘤坏死因子 

分 类 号：R965[医药卫生—药理学]