HPLC测定五味沙棘颗粒剂中异鼠李素的含量  

Determination of Isorhamnetine in Wuweishaji Granules by RP HPLC

作　　者：韩峰超[1] 沈君[1] 赵霓[1] 童丹[2] 李玉天 

机构地区：[1]沈阳市药品检验所,辽宁沈阳110001 [2]沈阳市药品监督管理局,辽宁沈阳110001

出　　处：《中成药》2000年第9期651-652,共2页Chinese Traditional Patent Medicine

摘　　要：目的 :采用反相高效液相色谱法测定五味沙棘颗粒剂中异鼠李素的含量。方法 :YMCODS分析柱 (4 .6× 15 0mm ,5 μm) ;甲醇 - 1.2 8%四丁基溴化胺 -醋酸缓冲溶液 pH 3.6 (4∶2∶2 )为流动相 ;流速 :1ml·min-1;检测波长 :370nm。结果 :线性范围 0 .0 96～ 0 .48μg(r =0 .9993) ,平均回收率 96 .9% ,RSD为 1.1% (n =6 )。 结论 :本法操作简便、准确可靠 。Objective: To determine the content of isorhamnetin in Wuweishaji Granules by RP HPLC. Methods: The HPLC system consisted of YMC ODS column (4.6×150mm, 5μ); mobile phase of methanol 1.28% tetrabutylammonium bromide HAc buffer pH 3.6 (4∶2∶2), with 1ml·min -1 of flow rate, detection at 370nm. Results: The linear range was 0.096～0.48μg(r=0.9993). The mean recovery was 96.9%, RSD=1.1%(n=6). Conclusion: This method is convinient, precise and reliable without intereference. It is suitable for preparation quility control.

关 键 词：高效蚁相色谱法 异鼠李素 五味沙棘颗粒剂 中药 

分 类 号：R286.0[医药卫生—中药学]