快速提取蓖麻基因组DNA不同方法的比较  

Comparison of Methods for Rapid Genomic DNA Extract from Castor Oil Plant

作　　者：张智勇[1,2,3] 倪娜[2] 李国瑞[2,3] 王超[2] 朱国立[1,2,3] 何智彪[1,2] 贾娟霞[1] 莫德乐吐[1] 乔文杰[1,2] 陈永胜[2,3] 

机构地区：[1]通辽市农业科学研究院,内蒙古通辽028015 [2]内蒙古民族大学,内蒙古通辽028043 [3]内蒙古高校蓖麻工程中心,内蒙古通辽028000

出　　处：《内蒙古农业科技》2013年第4期20-22,共3页Inner Mongolia Agricultural Science and Technology

基　　金：国家自然科学基金资助项目"蓖麻源库平衡规律探索"(31160262);教育部新世纪优秀人才支持计划资助项目"蓖麻内源毒素的基因沉默研究"(NCET-08-0870);公益性行业(农业)科研专项资助项目"蓖麻产业技术研究及实验示范"(201003057)

摘　　要：探讨最适于PCR试验的快速高效蓖麻DNA提取方法。采用改良CTAB法、改良SDS法和植物基因组提取试剂盒法提取蓖麻嫩叶片总DNA,对所提DNA进行分光光度计及琼脂糖凝胶电泳法测定其纯度与浓度,并以其为模板进行ISSR-PCR分析。改良SDS法与改良CTAB法所提取的DNA浓度较低,纯度不高,用时较长,操作较难;试剂盒法提取的DNA浓度较高,纯度较好,操作简单用时少,费用较高,三种方法所提DNA在ISSR-PCR均能扩增出条带。在所用材料较少、试验次数较少的情况下,首选试剂盒提取DNA的方法,该法快速且提取成功率高。Study on a quick and effective method of castor DNA was suitable for the ISSR amplification experiment. Genomic DNA was extracted from castor tender leaf with improved CTAB method, improved SDS method and kit method, the purity and concentration was measured by spectrophotometer and agarose gel electrophoresis of the DNA, and takes it as the template for ISSR-PCR analysis. Result：Improved SDS method and modified CTAB method to extract the DNA concentration was low, the purity was not high, takes longer, operation is more difficult; Qiagen method to extract DNA concentration is higher, the purity was good, operation was simple and takes less, cost is higher. Three methods proposed in the ISSR DNA - PCR could amplify the stripe. Conclusion：Materials test under the condition of fewer,less preferred methods of extracting DNA kit, the fast and high success rate.

关 键 词：蓖麻 基因组DNA SDS法 CTAB法 

分 类 号：S565.6[农业科学—作物学]