牛心型α肌动蛋白基因的进化及表达研究  

Study on Evolutionary and Expression Pattern of Cardiac α-actin in Cattle

作　　者：张冉[1] 林浴霜[1] 

机构地区：[1]山东大学生命科学学院,山东省动物细胞与发育生物学重点实验室,山东济南250100

出　　处：《中国畜牧兽医》2013年第8期46-49,共4页China Animal Husbandry & Veterinary Medicine

基　　金：山东省良种工程项目:肉牛优异品质种质创新利用研究(SDLZ2010ZY03)

摘　　要：试验旨在对鲁西黄牛心型α肌动蛋白的进化及表达进行研究,为研究该基因的功能提供依据。试验采用生物信息学方法对心型α肌动蛋白的进化进行了分析,利用Western blotting和免疫荧光的方法分析了牛心型α肌动蛋白的表达模式。结果显示,心型α肌动蛋白除在鲁西黄牛心脏组织中有强表达外,在骨骼肌中也检测到该蛋白的表达。推测心型α肌动蛋白除在成体鲁西黄牛的心脏发育过程中起作用外,在骨骼肌发育过程中也起作用。The study on the evolution and expression pattern of cardiac a-actin in Luxi Huang cattle might provide some in- formation to understand the function of cardiac a-actin. We did the bioinformatics analysis to show the evolution information about the cardiac a-actin, and we did the Western blotting and immunofluoreseence experiments to show the expression pattern of cardiac a-actin. The results showed that the cardiac a-actin was expressed strongly in adult heart tissues, and at the same time it was expressed weakly in skeletal muscle. The expressed pattern suggested that the cardiac a-actin function was not only in heart development but in skeletal muscle development.

关 键 词：心型α肌动蛋白 表达模式 骨骼肌 心脏 

分 类 号：S823[农业科学—畜牧学]