酶定向进化方法的研究进展  

Research Progress on Directed Evolution of Enzymes

作　　者：张林[1] 朱小翌[1] 江明锋[1] 

机构地区：[1]西南民族大学生命科学与技术学院,动物遗传育种学国家民委-教育部重点实验室,四川成都610041

出　　处：《中国畜牧兽医》2013年第8期68-71,共4页China Animal Husbandry & Veterinary Medicine

基　　金：四川省青年科技基金(09ZQ026-011);教育部科学技术研究重点项目(210265);2011年度四川省学术和技术带头人培养资金资助(2011-1);新津县科技局科研项目(2011-2)

摘　　要：酶作为一种生物催化剂,已广泛应用于生活和生产中,但能直接应用于工业生产的天然酶并不多,因此,需要对酶进行人工改造。分子定向进化是在实验室模拟自然进化过程的一种技术,已广泛用于酶的改造中,并取得了较好的成绩。作者将简要介绍酶定向进化的原理、方法及应用,并展望其发展前景。As a biological catalyst, enzymes had been widely used in industrial production. But few of nature enzymes could be directly used for industrial production. So, nature enzymes needed be improved. Directed evolution was a molecular biology technique that allowed natural evolutionary processes to be mimicked in the laboratory, it was widely used to improve enzymes and achieved success. This article would be briefly described the methods and applications of directed evolution, meanwhile, the development of directed evolution was also prospected.

关 键 词：定向进化 化学诱变 易错PCR DNA改组 随机引发体外重组 

分 类 号：Q814.9[生物学—生物工程]