亚硒酸钠对游离脂肪酸致肝细胞凋亡和线粒体膜电位降低的保护作用  

Protective Effect of Sodium Selenite on Hepatocyte Apoptosis and the Decrease of Mitochondrion Transmembrane Potential Induced by Free Fatty Acids

作　　者：张鹏春[1] 陈丽君[1] 范瑞威[1] 郭苗苗[1] 郭志侯[1] 张伟倩[1] 张志刚[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国畜牧兽医》2013年第8期83-88,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金青年项目(31101868);黑龙江省青年科学基金(QC2010057);中国博士后科学基金特别资助(2012T50302);东北农业大学大学生科创基金(2011056);黑龙江省普通高等学校新世纪优秀人才计划项目(1253-NCET-007)

摘　　要：为研究亚硒酸钠对游离脂肪酸(free fatty acid,FFA)致体外培养大鼠肝细胞凋亡和线粒体膜电位降低的保护作用,本试验通过胶原酶两步灌流法分离培养肝细胞,在细胞培养液中添加FFA和亚硒酸钠(0.1μmol/L)测定细胞的凋亡情况、细胞功能和线粒体膜电位。结果表明,随着培养液中FFA浓度的升高,细胞凋亡加重,肝细胞功能受到影响,线粒体膜电位降低且FFA的这种作用呈剂量依赖性;亚硒酸钠能抑制由FFA引起的肝细胞的上述变化。The objective of this study was to define the effect of sodium selenite on hepatocyte apoptosis and the decrease of mitochondrion transmembrane potential induced by free fatty acids （FFA）＊. Primary rat hepatocytes were obtained with two step collagenase perfusion method. We estimated hepatocyte apoptosis, cell function and mitoehondrion transmembrane poten tial in FFA treated group with or without sodium selenite （0.1μmol/L）. Our data indicated that FFA changed dose-depend ently hepatocyte apoptosis, hepatocyte function and mitochondrion transmembrane potential, whereas the presence of sodium selenite reversed these alternations.

关 键 词：亚硒酸钠 游离脂肪酸 肝细胞凋亡 肝细胞功能 线粒体膜电位 

分 类 号：S816.15[农业科学—饲料科学]