香附酮抑制鸡致病性大肠杆菌诱导肺泡Ⅱ型上皮细胞炎症作用的研究  

Inhibitory Effect of Cyperone on Inflammation of Chicken Alveolar Epithelial Type Ⅱ Cells Induced by APEC

作　　者：张立艳[1] 郭勋[1] 夏方[1] 胡汐柔[1] 秦倩倩[1] 张翠[1] 宋舟[1] 申海清[1] 伊鹏霏[1] 韦旭斌[1] 付本懂[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062

出　　处：《中国畜牧兽医》2013年第8期92-96,共5页China Animal Husbandry & Veterinary Medicine

基　　金：吉林大学科学前沿与交叉学科创新项目(20120331);吉林大学博士科研启动基金项目(20070302)

摘　　要：本试验通过建立鸡致病性大肠杆菌O78(APEC-O78)感染的鸡肺泡Ⅱ型上皮细胞的体外炎症模型,研究了不同浓度香附酮对TNF-α、IL-10分泌及TLR-4和TLR-5mRNA表达水平的影响。结果显示,0.5μg/mL香附酮能显著降低TNF-α的分泌水平(P<0.05),同时1μg/mL香附酮能极显著促进IL-10的分泌(P<0.01)。0.1、0.5和1μg/mL香附酮均能极显著降低TLR-4mRNA的表达水平(P<0.01),而不影响TLR-5mRNA的表达水平(P>0.05)。结果表明,香附酮可能通过TLR-4受体信号通路,抑制APEC-O78感染的鸡肺泡Ⅱ型上皮细胞促炎因子的产生,增加抑炎因子的产生,进而发挥其抗炎作用。Abstract：The effects of cyperone on chicken alveolar epithelial type Ⅱ cells infected by APEC-O78 were evaluated through determining TNF-α, IL-10, TLR-4 and TLR-5 in vitro. The results showed that 0.5 μg/mL cyperone significantly decreased the secretion of TNF-a（P〈0.05）. 1 t^g/mL cyperone extremly significantly increased IL-10 secretion（P〈0.01）. Further- more, 0.1, 0.5 and 1 /zg/mL cyperones all extremly significantly decreased the expression of TLR-4 mRNA（P〈0.01）, but had no effect on the expression of TLR 5 mRNA（P〈0.05）. The results indicated that cyperone might inhibit the production of inflammatory cytokines of chicken alveolar epithelial type Ⅱ cells induced by APEC-O78 through blocking TLR-4 signaling pathway.

关 键 词：香附酮 鸡大肠杆菌O78(APEC-O78) 鸡肺泡Ⅱ型上皮细胞 炎症 信号通路 

分 类 号：S852.614[农业科学—基础兽医学]