利用微卫星标记研究贵州矮马和伊犁马的遗传多样性  

Evaluation on the Genetic Diversity of Guizhou Pony and Ili Horse Using Microsatellite Markers

作　　者：雷红梅[1] 王嘉福[1,2] 田松军 王荣明 黄勇 冉雪琴[2] 

机构地区：[1]贵州大学农业工程重点实验室,贵州贵阳550025 [2]贵州大学动物科学学院,贵州贵阳550025 [3]贵州省紫云县农业局,贵州紫云561600

出　　处：《中国畜牧兽医》2013年第8期116-122,共7页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省农业攻关项目(黔科合NY字2009-3068;黔科合NY字[2012]3009号);贵州省农业动植物育种专项(黔农育专字2009-021号);贵州省科技创新人才团队建设专项(黔科合人才团队2009-4006);贵州大学研究生创新基金(校农研2012010)

摘　　要：试验通过9个微卫星座位对贵州矮马和伊犁马2个品种共84匹马进行了遗传多样性检测,以8%非变性聚丙烯酰胺凝胶电泳分离PCR扩增产物,并用银染法显色,计算各微卫星位点的等位基因频率、有效等位基因数(Ne)、杂合度(H)和多态信息含量(PIC)。结果表明,9个微卫星座位中COR059检出的变异最大,AHT040的变异最小;伊犁马的Ne、H和PIC的平均值高于贵州矮马;2个群体的平均遗传分化系数为0.041。说明2个品种都有丰富的遗传多样性,贵州矮马的基因较伊犁马更为纯合。Nine microsatellite loci were chosen to detect the genetic diversity of Guihzou pony and Ili horse in this experi ment. Polymerase chain reaction （PCR） products were detected by 8% native polyaerylamide gel electrophoresis in silver stai ning protocol. Allele frequency numbers of effective alleles （Ne）,heterozygosity （H） and polymorphism information contents （PIC） for each microsatellite locus were calculated by POPGENE software. The greatest variation was found from COR059, while the lowest was from ATH040 in all of 9 microsatellite loci. Mean numbers of Ne, H and PIC in Ill horse were higher than that in Guizhou pony. Mean coefficient of gene differentiation （GsT） of whole breeds was 0. 041. It suggested that the gene of Guizhou pony was more homozygous than that of Ill horse, and both of them showed plenty of genetic variation.

关 键 词：微卫星标记 遗传变异 贵州矮马 伊犁马 

分 类 号：S821[农业科学—畜牧学]