四川道地产区乌头药材不同部位6种生物碱含量对比研究  

Difference evaluation of three kinds of root of Aconitum carmichaelii in Sichuan based on UPLC analysis of six alkaloids and chemometrics

作　　者：钱长敏[1,2] 宋兆辉[2] 张兰兰[2] 周水平[2] 冯锋[1] 

机构地区：[1]中国药科大学,江苏南京210009 [2]天津天士力集团研究院现代中药研究所,天津300410

出　　处：《中国中药杂志》2013年第17期2761-2767,共7页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2013zx09402202)

摘　　要：目的:研究四川道地产区6种乌头生物碱在不同部位的分布差异,比较母根、子根基于化学成分的区别。方法:采用超高效液相色谱法(UPLC)测定19批四川道地乌头药材母根、子根、须根中6种乌头生物碱含量,并结合SPSS统计软件对数据进行显著性差异、聚类分析和主成分分析研究。结果:6种乌头生物碱在3个部位的分布差异各不相同。母根、子根中苯甲酰新乌头碱、新乌头碱含量有显著性差异,为两者的差异性指标成分。聚类分析、主成分分析也将供试样品分成母根、子根两大类。结论:母根、子根的物质基础有差别,可为临床正确使用提供参考。An ultra performance liquid chromatography （UPLC） method was established and validated to simultaneously deter-mine the contents of six aconitum alkaloids in mother, daughter and fibrous roots of 19 batches of Aconitum carmichaelii from Sichuan province. The separation of the six alkaloids was achieved on a ACQUITY UPLC BEH Cls （2. 1 mm × 100 mm, 1.7 μm） column at 40 ℃ with a mobile phase consisting of acetonitrile in 30 mmol · L^-1 ammonium acetate buffer solution（ adjusted to pH 10.0 with aqueous ammonia） in gradient mode. The data and plots showed that the six aconitum alkaloids have different distributions. Four aconitum alka-loids were almost same in mother and daughter root except benzoylmesaconine and mesaconitine, while the fibrous root differed from the other two roots. The comparisons of significant differences of six aconitum alkaloids between the mother and daughter roots definitely demonstrated that benzoylmesaconine and mesaconitine were the representative components. The 38 detecting samples were classified as two clusters by hierarchical clustering analysis （HCA） and principle component analysis （PCA）, the results indicated that the mother root was different from the daughter root on chemical material basis. The study might contribute to the reasonable clinical application of A. carmichaelii.

关 键 词：UPLC 乌头 母根 子根 乌头生物碱 区别 

分 类 号：R284[医药卫生—中药学]