HPLC双波长法同时测定ZJHX橡胶膏中三七皂苷R_1,人参皂苷Re,Rg_1,Rb_1和血竭素的含量  

Simultaneous content determination of notoginsenoside R_1,ginsenoside Rg_1,Re,Rb_1 and dracorhodin in ZJHX rubber paste by double wavelength HPLC

作　　者：胡琴[1,2] 孙娥[1] 徐凤娟[1,2] 张振海[1] 贾晓斌[1] 

机构地区：[1]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,江苏南京210028 [2]江苏大学药学院,江苏镇江212013

出　　处：《中国中药杂志》2013年第17期2793-2797,共5页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2008BAI53B072)

摘　　要：采用HPLC双波长法同时测定ZJHX橡胶膏中三七皂苷R1，人参皂苷Re，Rg1，Rb1和血竭素的含量，选用乙腈．水作为流动相进行梯度洗脱，流速1．0mL·min^-1，进样量10μL，柱温35℃，检测波长分别为203nm（皂苷类），440nm（血竭素）。结果显示，三七皂苷R1，人参皂苷Re，Rg1，Rb1和血竭素均能达到基线分离，线性范围分别为0．251～5．020，0．520～10．400，0．251～5．010，0．505～10．100，0．160—3．270μg，R^2分别为0．9998，0．9999，0．9997，0．9998，0．9999，各成分在其线性范围内均呈现良好的线性关系，加样回收率99．39％-100．5％。所建立的HPLC双波长法操作简便、结果准确、重复性好，可用于ZJHX橡胶膏的质量控制。Notoginsenoside R1 , ginsenoside RgI , Re, Rb1 and dracorhodin from ZJHX rubber paste were analyzed simultane-ously by HPLC, with aeetonitrile-water as the mobile phase for gradient elution at a flow rate of 1.0 mL·min^-1. The column tempera-ture was 35 ℃ and the sample size was 10 μL. The detection wavelength was set at 203 nm for ginsenoside and 440 nm for draeorhod-in, respectively. The results showed that all of notoginsenoside R1 , ginsenoside Rgl, Re, Rb1 and dracorhodin could be were separated well by baseline, with the linear ranges of 0. 251-5.020 μg （ R^2 = 0. 999 8 ）, 0. 520-10. 400 μg （ R^2 = 0. 999 9 ）, 0. 251-5.010 μg （R^2 =0. 999 7）, 0. 505-10. 100 μg （R^2 =0. 999 8） and 0. 160-3. 270 μg （R^2 =0. 999 9）, respectively. Each component showed a good linear relationship, with the average recoveries ranging from 99.39% to 100.5%. The established method was so simple, accu-rate and highly reproducible that it could be used for quality control of ZJHX rubber paste.

关 键 词：HPLC 三七皂苷R1 人参皂苷Rg1 人参皂苷RE 人参皂苷Rb1 血竭素 

分 类 号：R286.0[医药卫生—中药学]