人参皂苷Rg1在造血干／祖细胞连续移植中对抗细胞衰老的作用研究  

Study on anti-aging effect of ginsenoside Rg_1 in serial transplantation of hematopoietic stem cells and progenitor cells

作　　者：周玥[1,2] 王建伟[1] 姜蓉[1] 姚欣[1] 杨斌[1] 蔡世忠[1] 刘俊[1] 刘典锋[1] 王亚平[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室,组织学与胚胎学教研室,重庆400016 [2]云南省大理学院组织胚胎学教研室,云南大理671000

出　　处：《中国中药杂志》2013年第17期2848-2853,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973818,81202785,30970872);重庆市科委自然科学基金重点项目(CSTC,2009BA5038)

摘　　要：目的:探讨人参皂苷Rg1在造血干/祖细胞(HSC/HPC)连续移植中对抗细胞衰老的作用。方法:免疫磁性分选法分离纯化的雄性供体小鼠Sca-1+HSC/HPC连续移植3代构建HSC/HPC衰老体内模型。60Coγ射线致死剂量辐射雌性受体鼠后分4组:对照组、衰老模型组、Rg1治疗衰老组、Rg1延缓衰老组。荧光定量PCR检测受体鼠骨髓细胞Y染色体(Sry基因)表达,确定受体鼠重建造血细胞来源;观察受体鼠存活时间及外周血血象指标的恢复,确定受体鼠造血功能重建情况及Rg1促进造血恢复情况;造血祖细胞混合性集落(CFU-Mix)培养,细胞周期分析和衰老相关β-半乳糖苷酶(SA-β-Gal)染色分析Sca-1+HSC/HPC衰老的生物学特点及Rg1体内调控Sca-1+HSC/HPC衰老的作用。结果:雌性受体鼠重建造血细胞来源于雄性供体鼠;连续移植后受体鼠30 d存活率明显降低,外周血象恢复延缓,Sca-1+HSC/HPC出现细胞衰老特征:G0/G1期细胞比例及SA-β-Gal染色阳性率增高,CFU-Mix数量下降。与同代衰老模型组相比,Rg1治疗衰老组及Rg1延缓衰老组受体鼠30 d存活率,WBC,HCT,PLT增高,Sca-1+HSC/HPC G0/G1期细胞比例、SA-β-Gal染色阳性率下降,CFU-Mix数量升高。Rg1延缓衰老组变化较Rg1治疗衰老组明显。结论:人参皂苷Rg1在HSC/HPC连续移植过程中具有延缓和治疗Sca-1+HSC/HPC衰老的作用。Rg1延缓衰老作用优于Rg1治疗衰老作用。Objective： To investigate the anti-aging effect of ginsenoside R1 in serial transplantation of hematopoietic stem cells and progenitor cells. Method： HSC/HPC aging model in vivo was established through the Sca-1 ^＋ HSC/HPC serial transplantation of male donor mice that had been separated and purified by the magnetic-activated cell sorting method. The female recipient mice that had been radiated with lethal dose of 60^ Co γ ray were divided into four groups ： the control group, the aging group, the Rgl-treated aging group and the Rglanti-aging group. The expression of Sry genes in bone marrow cells of recipient mice was analyzed by fluorescence quantitative PCR, in order to determine the source of hematopoietic reconstruction ceils, observe the survival time and the recovery of the hematology of peripheral blood, and study the reconstruction of the hematopoietic function of recipient mice, the hematopoietic re-covery promoted by Rgl, the culture of CFU-Mix of hemopoietic progenitor cells, the cell cycle analysis and aging-related SA-β-Gal staining analysis on biological characteristics of Sca-1 ^＋ HSC/HPC aging, and the effect of Rg1 in vivo regulation on Sca-1 ^＋ HSC/HPC aging. Result： The hematopoietic reconstruction cells of female recipient mice were derived from male donor mice. With the serial transplantation, the 30-day survival rate and the hematology in peripheral blood of recipient mice decreased. Sca-1 ^＋HSC/HPC showed aging characteristics ： the ratio of cells in G0/G1 phase and the positive rate of SA-β-gal staining increased, whereas the number of CFU-Mix decreased. Compared with the aging group of the same generation, Rg1-treated aging group and Rg1 anti-aging group showed higher 30-day survival rate and WBC, HCT, PLT and CFU-Mix, and lower cell ratio in Sca-1 ^＋ HSC/HPC G0/G1stage and positive rate of SA-β-gal staining. The Rg1 anti-aging group showed more significant changes than the Rg1-treated aging group. Conclusion： Ginsenoside Rgl has the effect of delaying and treat

关 键 词：人参皂苷RG1 造血干 祖细胞 抗衰老 连续移植 

分 类 号：R285.5[医药卫生—中药学]