藏药材“白花刺参”抑制NO生成作用的谱效相关分析  

Spectrum-effect correlation analysis of traditional Tibetan medicine ″Morina nepalensis″ on nitric oxide production inhibition

作　　者：罗培[1] 刘圆[2] 吕露阳[2] 张志锋[2] 

机构地区：[1]中药质量研究国家重点实验室澳门科技大学 [2]西南民族大学少数民族药物研究所,成都四川610041

出　　处：《中国中药杂志》2013年第17期2882-2885,共4页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2012BAI27B07);澳门特别行政区科学技术发展基金项目(073/2011/A3)

摘　　要：目的:研究白花刺参HPLC-ELSD的化学指纹与抗一氧化氮(NO)生成作用的相关性,为确定抗炎活性成分推荐质量控制指标提供依据。方法:色谱柱为Eclipse XDB C18柱(4.6 mm×150 mm,5μm);流动相为水-乙腈,梯度洗脱,体积流量为1 mL·min-1;柱温35℃;ELSD漂移管温度为104℃;N2气体流速为2.8 L·min-1;进样量10μL。测定10批白花刺参的HPLC-ELSD指纹图谱和对脂多糖诱导巨噬细胞生成NO的抑制作用,采用相似度分析和偏最小二乘法对HPLC-ELSD指纹图谱与抗NO生成作用进行相关分析。结果:在10批药材色谱指纹图谱中,相似度均在0.90以上,确定15个共有峰,其中7个色谱峰与抗炎活性呈显着正相关,可被选作质量控制指标。结论:该指纹图谱检测方法方便,重复性好,可用于白花刺参药材质量控制和抗炎活性评价的实验依据。Objective ： To establish an HPLC-ELSD fingerprint of the whole herbs of Morina nepalensis and perform the corre-lation analysis of chemical components of the herb and nitric oxide （NO） production inhibition. Method ： HPLC-ELSD assay was per-formed to evaluate 10 batches of M. nepalensis herbs. The chromatographic conditions were as following： Eclipse XDB Cls column （4.6 mm × 150 mm,5 μm）, water （A） and acetonitrile （B） as a gradient mobile phases, flow rate 1.0 mL · min^-1, and column temperature at 35 ℃. Evaporative light-detection conditions： atomization temperature at 104 ℃, the flow rate of N2 2.8 L · min^-1 and 10 μL sample injection. Chromatographic fingerprint was developed, and the inhibition activity of production of NO in lipopolysaccha-ride-induced macrophages was also analyzed. The similarity and correlation analysis between the HPLC-EI_SD fingerprints and NO pro-duction inhibition were carried out by PLS method. Result： The common mode for M. nepalensis herb fingerprint was established, in-cluding 15 common characteristic peaks. Among them,7 peaks were positively correlated with the NO production inhibition. According to the assessment on the similarity of 10 batches of samples, a similarity of over 0.90 were shown in HPLC-ELSD fingerprint and all samples were separated into two groups. Conclusion： This method can be used to assess the quality of M. nepalensis, which provides a reliable method for scientific assessment and quality control.

关 键 词：白花刺参 HPLC—ELSD 指纹图谱 相关分析 抗炎活性 

分 类 号：R29[医药卫生—民族医学]