RP-HPLC法测定莱菔子中芥子酸乙酯的含量  

Determination of Sinapic Acid Ethyl Erther in Semen Raphani by HPLC

作　　者：郑德[1] 周洪雷[1] 李民[1] 蒋海强[1] 李运伦[2] 张玲[1] 朱立俏[1] 史玉霞[1] 黄真[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学附属医院,山东济南250014

出　　处：《辽宁中医杂志》2013年第9期1883-1884,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：山东省自然科学基金项目(ZR2010HM017);山东省教育厅科技计划项目(J105LL05);国家科技重大专项"重大新药创制"-"山东省重大新药创制中心建设"(2009ZX09301-013);国家自然科学基金(81273700)

摘　　要：目的：建立莱菔子中芥子酸乙酯的高效液相色谱测定方法。方法：RP-HPLC法测定。HypersilODS2色谱柱,乙腈-水为流动相,检测波长为320nm。结果：芥子酸乙酯含量在4.88～78.08μg范围内线性良好,回收率为101.45%,RSD为1.97%。结论：该方法简便灵敏,适合莱菔子的质量控制。Objective：To establish a method for determing polysaccaride content of Sinapic acid ethyl erther by HPLC. Meth- ods ： HPLC method was selected to determine the content. The separation was performed on an analytical Hypersil ODS2 column. The mobile phase was acetonitril - water. The UV detector was set at 320 nm. Results： Linear range of determination of Sinapic acid ethyl erther was 4.88-78.08 μg, the mean recovery was 101.45% for Sinapic acid ethyl erther, RSD 1.97%, （n = 6）. Conclusion：The method is sensitive, accurate and with good reproducibility.

关 键 词：莱菔子 芥子酸乙酯 HPLC 含量测定 

分 类 号：R927.2[医药卫生—药学]