纤维素^(13)C同位素示踪法研究纤维素与木素连接方式  

Analysis of the Association between Cellulose and Lignin by Carbon 13 Tracer Method

作　　者：向松明[1] 谢益民[1] 杨海涛[1] 姚兰[1] 

机构地区：[1]湖北工业大学制浆造纸工程研究所,湖北武汉430068

出　　处：《光谱学与光谱分析》2013年第9期2488-2491,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(31070521;30901140)资助

摘　　要：为了阐明裸子植物中纤维素与木素之间的连接方式,从多糖的角度分析糖单元与木素苯丙烷结构单元之间的共价键连接方式,合成了带6-13 C标记的纤维素前驱物尿苷二磷酸葡萄糖,并将其与PAL酶的抑制剂AOPP及外源性木素前驱物松柏醇-β-D-葡萄糖苷一起投入生长中的银杏植物体内。碳13丰度检测得知纤维素在细胞壁的沉积主要是从在初生壁开始,高分辨率固体核磁13 C NMR分析证实了纤维素6位碳与木素以苯甲醚键等方式连接。In order to further understand the association between cellulose and lignin in gymnosperm plant,carbohydrate part of the lignin-carbohydrate complexes was analyzed.Cellulose precursor i.e.（6-^13 C）uridine diphoshphate glucose,was synthesized,and injected into a living ginkgo tree with lignin inhibitor AOPP and exogenous lignin precursor.The results from the determination of ^13 C abundance indicate that the deposition of cellulose in the cell wall is very fast and effective in the primary wall.The analysis of high resolution solid nuclear magnetic ^13 C NMR spectra confirms that the bonds between C6position of glucose units in cellulose andα-carbons of lignin side chain are benzyl ether linkage.

关 键 词：纤维素 13C标记 CP/MAS NMR 木素-碳水化合物复合体 

分 类 号：TS711[轻工技术与工程—制浆造纸工程]