利用四引物扩增受阻突变体系PCR技术检测水稻低直链淀粉含量基因Wx-mq  

Detection of Wx-mq Gene for Low-amylose Content by Tetra-primer Amplification Refractory Mutation System PCR in Rice

作　　者：陈涛[1] 骆名瑞[1] 张亚东[1] 朱镇[1] 赵凌[1] 赵庆勇[1] 周丽慧[1] 姚姝[1] 于新[1] 王才林[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心,南京210014

出　　处：《中国水稻科学》2013年第5期529-534,共6页Chinese Journal of Rice Science

基　　金：江苏省农业科技自主创新基金资助项目[CX(11)4021];江苏省科技支撑计划资助项目(BE2011302);农业部超级稻育种与示范项目;现代农业产业技术体系建设专项资金资助项目(CARS-01-47)

摘　　要：根据Wx-mq基因存在的单核苷酸变异,利用四引物扩增受阻突变体系PCR(Tetra-primer ARMS-PCR)的方法设计特异引物,对12个水稻品种(或品系)以及武育粳3号(不含Wx-mq基因)/关东194(含Wx-mq基因)的F2分离群体进行扩增,依据其PCR产物的带型,可以准确区分出Wx-mq基因纯合、非Wx-mq基因纯合以及杂合基因型三种类型,其电泳结果与成熟后对胚乳外观特性鉴定完全一致。因此,作为一种简单、低成本的实用技术,四引物ARMS-PCR可以广泛用于Wxmq基因水稻的资源鉴定和分子标记辅助育种。To improve the efficiency of selection for Wx-rnq gene in breeding, we designed specific primers by tetra- primer Amplification Refractory Mutation System PCR （ARMS-PCR） method, according to the single nucleotide mutation in Wx-mq gene to analyze 12 rice varieties （or lines） and the Fz segregation population derived from the cross of low amylose content variety Kanto 194 （with Wx-mq gene） and normal variety Wuyujing 3（without Wx-rnq gene）. The results of PCR detection indicated that three genotypes including the homozygous genotype with Wx-mq, the homozygous genotype without Wx rnq and the heterozygous genotype could be clearly distinguished according to band types, and it was completely consistent with the result of phenotypic analysis. Therefore, as a simple and low-cost technique,the tetra-primer ARMS PCR could be widely used to identify rice resources with Wx-rnq gene and applied in molecular marker-assisted breeding.

关 键 词：水稻 低直链淀粉含量 Wx—mq基因 四引物扩增受阻突变体系PCR 

分 类 号：S511[农业科学—作物学]