BARF1基因对鼻咽癌细胞NF-κB信号通路及bcl-2基因表达的影响  

Epstein-Barr virus-encoded BARF1 gene upregulates bcl-2 expression through activating NF-κB signaling pathway in human nasopharyngeal carcinoma cells

作　　者：徐美琴[1] 李友琼[2] 张雪怡[1] 褚福营[1] 张宇琼[1] 陈巧林[1] 成静[1] 周天戟[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013 [2]广西壮族自治区人民医院检验科,广西南宁530022

出　　处：《江苏大学学报（医学版）》2013年第2期137-139,184,共4页Journal of Jiangsu University：Medicine Edition

基　　金：江苏大学高级技术人才科研启动基金资助项目(06JDG011)

摘　　要：目的:探讨EB病毒编码的BARF1基因对鼻咽癌细胞NF-κB信号通路活性及bcl-2基因表达的影响。方法:以pSG5空载体转染鼻咽癌细胞(CNE1-SG)为对照,分别采用荧光抗体染色法和蛋白质印迹法检测稳定表达BARF1的鼻咽癌细胞系CNE1(CNE1-BARF1)中NF-κB蛋白的核转位和Bcl-2蛋白的表达变化。结果:与CNE1-SG相比,CNE1-BARF1细胞中发生明显的NF-κB核转位,同时Bcl-2蛋白表达量显著增加(t=3.89,P<0.05)。采用NF-κB的特异性抑制剂Bay11-7082处理后,NF-κB蛋白的核转位明显减少,同时Bcl-2蛋白表达量显著下降(t=3.13,P<0.05)。结论:BARF1基因通过激活NF-κB信号通路上调鼻咽癌细胞中bcl-2基因的表达。Objective： To investigate the effect of Epstein-Barr virus-encoded BARF1 gene on the nuclear translocation of NF-κB and bcl-2 expression in human nasopharyngeal carcinoma cells.Methods： Fluorescence antibody staining and Western blot were separately performed to analyze the nuclear translocation of NF-κB and bcl-2 expression in BARF1-stably-expressing human nasopharyngeal carcinoma cell line CNE1（CEN1-BARF1） under the control of pSG5 vector transfected one（CEN1-SG）.Results： Compared with the control,the nuclear translocation of NF-κB was significantly induced,and bcl-2 expression was significantly increased in CEN1-BARF1.And both were blocked by the specific inhibitor of NF-κB Bay11-7082.Conclusion： BARF1 gene upregulated bcl-2 expression through activating NF-κB signaling pathway in human nasopharyngeal carcinoma cells.

关 键 词：EB病毒 BARF1 鼻咽癌 NF-ΚB BCL-2 

分 类 号：R373.1[医药卫生—病原生物学] R739.6[医药卫生—基础医学]