Ⅰ-69杨与小叶杨杂交种胚培养研究  

Researches on Embryo Culture of Hybrids Between Populus deltoides cv.“Lux”and Populus Simonii

作　　者：胡超 汪建亚 王瑞文[2] 许红霞 汪明 于静[3] 

机构地区：[1]湖北省林业厅林木种苗管理总站,武汉430079 [2]湖北省林业科学研究院,武汉430075 [3]棕榈园林股份有限公司风景园林研究院,上海200443

出　　处：《湖北林业科技》2013年第4期14-20,共7页Hubei Forestry Science and Technology

摘　　要：本研究利用组织培养技术对进行培养挽救,以为了提高育种成功率。试验结果表明:外植体的最佳消毒方法为75%酒精消毒30 s后在0.1%升汞处理8 min,幼胚的成活率为90%。随着外植体消毒时间增加,幼胚成活率先上升后下降。幼胚最佳萌发培养基为1/2MS+BA0.1 mg.L-1+NAA0.01 mg.L-1。最佳继代增殖培养基为MS+BA0.1 mg.L-1+NAA0.01 mg.L-1,增殖系数达到6.33。对不定芽增殖最有利条件为:pH值6.0、琼脂浓度6 g.L-1和蔗糖浓度30 g.L-1。最佳生根培养基为1/2MS+IBA0.2 mg.L-1,生根率为93.2%。In this study, tissue culture technology was used to rescue immature embryo of hybrid from artificial crossing between Populus deltoides cv. ＂Lux＂and Populus simonii in order to improve breeding efficiency. The study showed that the optimal sterilizing methods of explants were dipping to 75% Ethanol for 30 s followed by treating with 0.1% HgCl2 for 8 min,and the survival rate of immature embryo was 90%. With increased time in explants sterilization, embryo survival rate increased and then decreased. 1/2 MS ＋NAA0. 01 mg · L^-1 ＋BA0. 1 mg · L^-1 was optimal germination medium of immature embryo. MS＋ NAA 0.01 mg · L^-1 ＋BAO. I mg · L^-1 were optimal proliferation medium. The Proliferation coefficient was 6.33. The optimum culture medium for proliferation contained 30 g · L^-1 sucrose, pH6.0,6 g· L^-1 ar- gar. 1/2MS＋IBA0.2 mg · L^-1 were the optimum rooting medium, and the rooting percentage was 93.2%.

关 键 词：Ⅰ-69杨 小叶杨 萌发培养基 继代增殖培养基 生根培养基 

分 类 号：S722.34[农业科学—林木遗传育种]