花梨木组织培养外植体消毒方法初步探讨  

The Preliminary Study in Disinfection Method of Explants of Dalbergia odofifera in Tissue Culture

作　　者：林妃[1] 李敬阳[1] 常胜合[1] 唐粉玲[1] 王必尊[1] 

机构地区：[1]中国热带农业科学院海口实验站,海南省香蕉遗传改良重点实验室,海口570102

出　　处：《基因组学与应用生物学》2013年第4期522-525,共4页Genomics and Applied Biology

基　　金：国家青年项目(基于不同施肥水平的巴西香蕉形态及产量的定量关系研究)(31101123);海南省科技计划重点项目(培育健康优质香蕉苗技术的示范与推广)(ZDXM20110013);海南省自然科学基金(基于不同施肥水平的巴西香蕉形态及产量的相关关系研究)(311062)共同资助

摘　　要：针对在花梨木(Dalbergia odofifera T.Chen)组织培养过程中外植体的有效消毒方法进行研究。外植体消毒主要以2年生以上实生苗的茎段为材料,开展了茎段外植体次氯酸钠(5%,10%,15%)和升汞(0.05%,0.1%,0.2%)3个浓度水平的消毒试验。在此基础上,开展了外植体消毒的时间试验,次氯酸钠消毒时间为10min、20min、30min,升汞消毒时间为5min、10min、15min。结果表明:茎段外植体采用10%浓度的次氯酸钠消毒效果较好,最佳消毒方式75%的酒精浸泡2min,10%次氯酸钠消毒20min,该方法污染率最低、存活力最高。Explants were always infected in the tissue culture ofDalbergia odofiferc~ In this paper, the young stems from two-year-old seedings ofDalbe rgia odofife ra were selected as propagation material. Two disintection experiments with three concentrations of NaC10 （5%, 10% and 15%） and HgC% （0.1%, 0.2% and 0.3%） were carried out. On this basis, different time for treating explants were also considered, NaCIO＇S disinfection time for （10 min, 20 min, 30 min）, HgCI2＇S disinfection time for 5 min, 10 min, 15 mirr Results showed that the optimal concentration of NaC10 was 10%. The optimal disinfection schemes was that the stem explants were immersed in alcohol （75%） for 2 minutes, and then were immersed in NaC10 （10%） for 20 minutes with the lowest contamination rate and the highest survive rate.

关 键 词：花梨木 外植体 组织培养 消毒方法 

分 类 号：S792.99[农业科学—林木遗传育种]