硫酸氨基葡萄糖氯化钾对IL-1β诱导的兔软骨细胞的作用  

Effect of Glucosamine Sulfate Potassium Chloride on Rabbit Chondro-cytes Induced by IL-1β

作　　者：彭秀兰 沈妙根 马彪 张军 周亚楠 王玉峰 江慧星[3] 卢洋[4] 尹鸿萍[4] 

机构地区：[1]江苏双林海洋生物药业有限公司,江苏南通226000 [2]江苏雨润肉类产业集团有限公司,江苏南京210041 [3]南京中医药大学第三附院南京市中医院,江苏南京210000 [4]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《药物生物技术》2013年第4期293-296,共4页Pharmaceutical Biotechnology

基　　金：江苏省重大科技成果转化专项资金项目(No.BA2009082)

摘　　要：采用原代培养的兔关节软骨细胞,检测硫酸氨基葡萄糖氯化钾对IL-1β诱导造成的炎性反应的保护作用。加入0.2,2,20μmol/L硫酸氨基葡萄糖氯化钾及10 ng/mL IL-1β48 h后,测定蛋白聚糖酶(Aggrecanase-1,-2)和诱导型一氧化氮合酶(iNOS)mRNA表达量的变化。结果表明,硫酸氨基葡萄糖氯化钾对于兔软骨细胞无明显增殖和细胞毒作用。对于IL-1β诱导的软骨细胞,硫酸氨基葡萄糖氯化钾可抑制其聚蛋白聚糖酶Aggrecanase mRNA表达量,并可抑制NO释放以及iNOS mRNA表达,与IL-1β模型组相比有极显著差异。硫酸氨基葡萄糖氯化钾保护骨性关节炎的机制与可显著抑制由白介素类炎性因子导致的炎性反应相关。Primary cultured rabbit chondrocytes were used to study the protective effect of glucosamine sulfate potassium chloride on IL-1β induced inflammatory reaction.After being cultured with 0.2,2,20 μmol / L of glucosamine sulfate potassium chloride and 10 ng / mL of IL-1β for 48 hours,Aggrecanase-1,-2 and iNOS were tested in RNA level in different groups.The results showed that 0.2,2,20 μmol / L of glucosamine sulfate potassium chloride had no cytotoxic on rabbit chondrocytes.After 2,20 μmol / L glucosamine sulfate potassium chloride or 40 μmol / L glucosamine hydrochloride combined with 10 ng / mL IL-1β treated,the RNA level of aggrecanase-1,-2 and iNOS had significant differences from the IL-1β induced control group.The release of NO was also tested to have significant differences from the IL-1β induced control group.The mechanism may be related to IL-1β inducement of inflammatory reaction.

关 键 词：硫酸氨基葡萄糖氯化钾 骨关节炎 兔软骨细胞 蛋白聚糖酶 诱导型一氧化氮合酶 IL-1Β 

分 类 号：R965[医药卫生—药理学]