木犀草素对心肌细胞物理性及化学性缺氧/复氧损伤的作用  

Effects of Luteolin on Two Types of Hypoxia /Reoxygenation Injury in H9c2 Cardiomyocytes

作　　者：李梦雯[1] 于东升[1] 田友清[2] 柳军[1] 尚靖[1] 

机构地区：[1]中国药科大学新药筛选中心,南京210009 [2]江苏联合职业技术学院连云港中医药分院,江苏连云港222007

出　　处：《中国药学杂志》2013年第17期1449-1453,共5页Chinese Pharmaceutical Journal

基　　金：国家"重大新药创制"科技重大专项(2011ZX09401-007);国家科技支撑计划(2012BAI30B00);中央高校基本科研业务费专项基金(JKY2009025)

摘　　要：目的比较木犀草素对物理性及化学性缺氧/复氧引发H9c2心肌细胞损伤的影响,考察木犀草素对抗两种缺氧/复氧损伤作用。方法体外培养H9c2心肌细胞,以N2饱和缺氧箱及Na2S2O4为缺氧环境分别建立缺氧/复氧损伤模型。测定H9c2心肌细胞存活率、乳酸脱氢酶漏出量、丙二醛的生成量、超氧化物歧化酶以及活性氧的生成量、线粒体膜电位(△Ψm)的变化,考察木犀草素对H9c2心肌细胞缺氧/复氧损伤的影响。结果与模型组比较,木犀草素能显著增强缺氧/复氧损伤心肌细胞存活率,抑制乳酸脱氢酶漏出、丙二醛和活性氧的生成量,提高超氧化物歧化酶的活性(P<0.05或P<0.01),恢复细胞膜电位水平。结论木犀草素对缺氧/复氧心肌细胞损伤有明显的保护作用,能够有效维持抗氧化防御机制与自由基之间的平衡。OBJECTIVE To investigate and compare the effects of luteolin on H9c2 cardiomyocytes damaged by two types of hy- poxia/reoxygenation （H/R） injury, and to ensure its protective action against hypoxia/reoxygenation injury. METHODS The H9c2 cardiomyocytes were cultured, and H/R injury model were induced by N2 saturation hypoxia chamber or sodium dithionite （ Na2 S2 O4 ）. In both models, the viability of cardiomyocytes, LDH leakage, MDA levels, T-SOD activities, ROS levels in cells, and mitochondrial membrane potential （△ψm ） changes were assessed to evaluate the effects of luteolin on H/R injury in H9c2 cells. RESULTS Com- pared with control, luteolin significantly increased cell viability, T-SOD activity in H9c2 cardiomyocytes on H/R injury, and inhibited LDH leakage, MDA and ROS level, and improved △ψm （ P 〈 0. 05 or P 〈 0. 01 ）. CONCLUSION Luteolin has a protective effect against H/R injury in H9c2 cardiomyocytes, and may effectively sustain the balance between anti-oxidant defense and free radicals.

关 键 词：木犀草素 心肌细胞 缺氧复氧 活性氧 

分 类 号：R965[医药卫生—药理学]