张家口坝上高背鲫鱼RAPD遗传多样性研究  

Study on genetic polymorphism(RAPD) of high-backed Carassius auratus from Zhangjiakou Bashang region

作　　者：韩青动[1] 吴江立[2] 陈力 

机构地区：[1]河北农业大学海洋学院,河北秦皇岛066003 [2]河北大学生命科学学院,河北保定071002 [3]河北省海洋与水产科学研究院,河北秦皇岛066005

出　　处：《河北农业大学学报》2013年第4期101-104,共4页Journal of Hebei Agricultural University

摘　　要：采用RAPD技术从40个随机引物中筛选出17个引物对张家口坝上高背鲫鱼水泉淖群体的遗传多样性进行研究,结果共检测出71个位点,其中多态位点25个,多态位点比例(P)为35.2%,平均每个引物提供的标记为4.2个;利用NTsyspc2.1软件获得该水库野生鲫鱼群体20个个体间的遗传距离,个体间遗传距离(D)在0.007 2～0.140 7之间,平均为0.063 5;个体间遗传相似系数(S)在0.859 3～0.992 8之间,平均遗传相似系数为93.65%;研究表明,张家口坝上野生高背鲫鱼群体由于环境封闭,没有外来品种引起的杂交现象,基因纯和程度较高,种群内遗传多样性低。Random amplified polymorphic DNA （RAPD） technique was applied to detect the DNA polymorphism in high-backed Cauratus auratus from Zhangjiakou Bashang region. 17 ran- dom primers were used and 71 sites were generated, and there were 25 polymorphic among them, with a percentage of 35.2 %. The average label of each primer was 4.2. The genetic dis- tance matrix of Cauratus were calculated by using software NTsyspc2.1, the genetic distance （D） and genetic similarity（s） among the individuals were 0. 007 2--0. 140 7, 0. 859 3--0. 992 8 respectively. Result showed that, because of environmental sealing, no cross phenomenon caused by exotic breed, high-backed Cauratus auratus from Zhangjiakou Bashang region had a high gene pure level and low genetic diversity.

关 键 词：高背鲫鱼 随机扩增多态DNA 遗传多样性 

分 类 号：S932.4[农业科学—渔业资源]