利用敲除PLCζ基因的JM8细胞制备嵌合体小鼠  

Chimeric mice production by PLCζ-deletant JM8 cells

作　　者：潘晓靖[1] 王鹏博[1] 胡春超[1] 徐继强[1] 崔丽芳[1] 李相运[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071000

出　　处：《河北农业大学学报》2013年第4期105-109,共5页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金(C2012204047)

摘　　要：为了研究PLCζ对公鼠生殖能力的影响,试验采用显微注射的方法将敲除PLCζ基因的JM8胚胎干细胞注入8-细胞胚胎获得嵌合体小鼠,并通过微卫星分析检测公鼠生殖系嵌合的情况及能否通过生殖系传递。共移植562枚胚胎,出生小鼠127只,出生率为22.60%,其中成年的毛色嵌合体小鼠数为46只,嵌合率为36.22%,对10只成年嵌合体公鼠的12种组织(脑,心脏,膀胱,肺脏,肝脏,胃,肾脏,睾丸,胰腺,肠,肌肉,脾脏)进行检验,有4只小鼠睾丸发生了嵌合,说明通过显微注射获得了生殖系嵌合的公鼠,但是生殖系嵌合的公鼠和CD-1母鼠交配后没有生下毛色嵌合小鼠,说明这些公鼠没有生殖系传递的痕迹,表明敲除了PLCζ基因影响了嵌合体公鼠的生精能力。To explore the effect of PLCζ on reproductive capacity, chimeras were obtained by injecting the PLC^-deletant JM8 cells into 8-cell embryos. We detected whether these chimeras germline were derived partly from JM8 cells by microsatellite analysis. A total of 562 embryos were transplanted and 127 mice were born, which included 46 adult chimeras. The birth rate was 22.60%. and chimeric rate was 36.22%. We detected twelve tissues in 10 adult chimeras （brain, heart, bladder, lungs, liver, stomach, kidney, testes, pancreas, intestine, muscle, spleen） by microsatellite analysis . We found four testes chimeric mice and after mating with CD-1 female mice ,coat color chimeras were not born. The results indicated that the male mice whose germlines were derived partly from JM8 cells could not get JM8 fetus, which proved that the PLCζ influence the reproduction of male chimera mice.

关 键 词：显微注射 微卫星分析 PLCζ 嵌合体 

分 类 号：Q785[生物学—分子生物学]