大连地区1911例成年女性HPV亚型感染状况调查  

Investigation on infection status of HPV subtypes in 1 911 adult women in Dalian area

作　　者：张青[1] 杨真[1] 李小欢[1] 张春蕾[1] 崔益芬[1] 袁小林[1] 

机构地区：[1]大连大学附属中山医院中心实验室,辽宁大连116001

出　　处：《中国妇幼保健》2013年第25期4201-4204,共4页Maternal and Child Health Care of China

摘　　要：目的:调查大连地区成年女性生殖道人乳头瘤病毒(HPV)亚型的感染状况,为宫颈癌的筛查及防治提供理论依据。方法:采用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术对大连地区1 911例成年女性HPV的感染情况进行定性检测并加以分型。结果:在1 911例成年女性中,共检出HPV阳性374例,阳性率为19.57%,其中单一、双重、三重和四重基因型感染者分别为288例(占77.00%)、59例(占15.78%)、22例(占5.88%)和5例(占1.34%);单纯低危型感染67例(占17.91%),单纯高危型感染271例(占72.46%),低危型与高危型混合感染36例(占9.63%)。共检出21种基因型,包括高危型HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66、68、73、83和低危型HPV6、11、42、43基因型,在高危型中以HPV16检出率最高(阳性率为5.65%),其次为HPV58(阳性率为3.03%)和HPV52(阳性率为2.93%);在低危型中以HPV43检出率最高(阳性率为2.83%),其次为HPV6(阳性率为1.41%)和HPV42(阳性率为0.99%)。结论:大连地区1 911例成年女性HPV感染阳性374例,以单一基因型和单纯高危型感染为主;共检出21种基因型,高危型和低危型分别以HPV16和HPV43检出率最高。对成年女性开展HPV基因分型检测对于HPV感染和宫颈癌的诊断、治疗具有重要意义。Objective： To investigate the infection status of human papillomavirus （HPV） subtypes in adult women in Dalian area, provide a theoretical basis for screening, prevention, and treatment of cervical cancer. Methods ： DNA chip technique （ PCR expansion in vitro combined with DNA reserve dot blot） was used to qualitatively detect the infection status and subtypes of HPV in 1 911 adult women in Dalian area. Results： Among 1 911 adult women, 374 women were found with positive HPV, the positive rate was 19. 57%, the proportions of adult women with single, double, triple, and quadruple HPV genotypes accounted for 77.00% （288 women）, 15.78% （59 women）, 5.88% （22 women）, and 1.34% （5 women）, respectively; 67 adult women were found with simple low -risk HPV infection, accounting for 17. 91% ; 36 adult women were found with simple low - risk HPV infection combined with high - risk HPV infection, accounting for 9. 63%. A total of 21 kinds of genotypes were detected, including high - risk HPV subtypes （HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 83 ） and low - risk HPV subtypes （ HPV6, 11,42, 43 ） ; among the high - risk HPV subtypes, the detection rate of HPV16 was the highest （5.65%）, followed by HPV58 （3.03%） and HPV52 （2. 93% ） ; among the low - risk HPV subtypes, the detection rate of HPV43 was the highest （2. 83% ）, followed by HPV6 （ 1.41% ） and HPV42 （0. 99% ） . Conclusion： Among 1 911 a- dult women in Dalian area, 374 adult women were found with HPV infection, single genotype and simple high -risk infection was the main type; a total of 21 genotypes were detected, the detection rates of HPV16 and HPV43 were the highest among the adult women with high - risk and low- risk HPV infection. Conducting HPV genotyping among adult women has important significance in diagnosis and treatment of HPV infection and cervical cancer.

关 键 词：人乳头瘤病毒 基因分型 宫颈癌 DNA芯片技术 

分 类 号：R194[医药卫生—卫生事业管理] R737.33[医药卫生—公共卫生与预防医学]