代谢通量分析在酶合成过程中的应用研究进展  

Application of metabolic flux analysis in enzyme synthesis

作　　者：高振[1] 熊强[1] 徐晴[1] 宋萍[1] 李霜[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009

出　　处：《化工进展》2013年第7期1625-1628,共4页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金重点项目(20936002);国家973计划(2011CB710804)

摘　　要：代谢通量分析可以有效预测微生物的代谢状况,指导生物合成过程,已在多个领域得到成功应用。本文概括分析了三大代谢通量分析技术(基于物料平衡、基于同位素示踪技术以及基于基因组尺度规模网络)在工业生物技术领域的应用,阐述了相关技术的优势及局限性。在此基础上重点论述了基于物料平衡及同位素示踪这两类通量分析技术在酶合成领域的研究进展,分析了代谢通量分析应用于复杂代谢产物可能存在的问题,指出如何将信号转导、转录调控等信息纳入代谢网络将成为未来酶合成过程代谢分析的研究重点。Metabolic flux analysis can effectively predict the metabolic status of microorganism and guide the process of biosynthesis. It has been successfully applied in many fields. This paper summarizes the applications of three techniques of metabolic flux analysis in the field of industrial biotechnology, which were respectively based on material balance, isotope tracer techniques and genome-scale network. The advantages and limitation of these technologies are presented. Further the application of the two types of flux analysis techniques based on material balance and isotope tracer in the field of enzyme synthesis is discussed. The possible problem of the application of metabolic flux analysis in complex metabolites system is pointed out. At last, the paper indicates that how to bring the information, such as signal transduction, transcription regulation into metabolic network will become the future focus of metabolic analysis in enzyme synthesis.

关 键 词：代谢通量分析 物料平衡 同位素示踪 基因组规模代谢网络 酶 

分 类 号：Q814[生物学—生物工程]