介孔生物玻璃对人骨髓间充质干细胞早期黏附增殖行为的影响  

Influence of mesoporous bioactive glass on the promotion of the initial hBMSCs adhesion and proliferationx

作　　者：李扬[1] 刘怡卓[2] 郭亚平[2] 刘李娜[1] 朱振安[1] 

机构地区：[1]上海市骨科内植物重点实验室,上海交通大学医学院附属第九人民医院骨科,上海200011 [2]上海师范大学生命与环境科学学院,上海200234

出　　处：《生物骨科材料与临床研究》2013年第4期8-11,16,66,67,共7页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：上海教委重点学科建设基金(J50206);上海市科委基础研究重点项目(12JC1405600)

摘　　要：目的制备具有高度有序介孔结构的介孔生物玻璃,研究人骨髓间充质干细胞（hBMSCs）在其表面黏附、铺展、增殖等早期细胞生物学行为。方法通过溶胶凝胶法制备介孔生物玻璃（MBG）;接种人骨髓间充质干细胞（hBMSCs）于不同组材料表面共培养,采用四唑盐比色法（MTT）分析其对细胞增殖的影响,绘制增殖曲线;使用DAPI染核,在荧光显微镜下观测材料表面的细胞粘附情况;利用扫描电子显微镜（SEM）直接观察细胞在材料表面的铺展形态。结果在4h和12h,MBG能够显著促进hBMSCs在其表面的黏附;同时,第1~7d,MBG表面的细胞增殖数量显著多于没有介孔结构的普通生物玻璃（NBG）表面的细胞增殖数;在24h内的荧光显微镜观察下,MBG表面在早期有更多的hBMSCs黏附;在第1、4、7d SEM的观察下,MBG表面的细胞能够更均匀的分布并覆盖材料。结论具有有序介孔结构的介孔生物玻璃能够促进人骨髓间充质干细胞在其表面的早期黏附、铺展和增殖,具有更好的生物活性。Objective The main purpose of this study is to fabricate a well-ordered mesoporous bioactive glass（MBG） and to investigate its in vitro biocompatibility by using the hBMSCs.Method The proliferation of hBMSCs on the MBG and NBG sample discs was examined with a MTT assay.The adherent cells on the different sample surfaces were observed by using the DAPI staining method.The morphology of the hBMSCs was investigated by detecting the cells morphology and spreading under the SEM.Result Both the initial cell adhesion and proliferation were accelerated obviously on the MBG compared to the NBG after 4h and on days 1 to 7.At 7 day,most of the cells on the surfaces of MBG exhibit a fusiform-shaped morphology and evenly-distributed and almost completely covered the surface of MBG after the cells seeded,observed under the SEM.Conclusion The initial hBMSCs adhesion and proliferation are both accelerated obviously on the MBG,which indicates the MBG has an excellent biocompatibility compared with the NBG.

关 键 词：介孔生物玻璃 生物相容性 人骨髓间充质干细胞 骨组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]