人类白细胞抗原基因多态性与支气管哮喘儿童免疫球蛋白E水平的关系  

Association between human leukocyte antigen gene polymorphisms and total immunoglobulin E levels in children with asthma

作　　者：谢庆玲[1] 甄宏[1] 秦岭[1] 唐晓燕[1] 谭颖[1] 廖婷婷[1] 王敏[1] 肖国锋[1] 贺海兰[1] 

机构地区：[1]广西壮族自治区人民医院儿科,南宁530021

出　　处：《中华实用儿科临床杂志》2013年第16期1227-1230,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：广西壮族自治区卫生厅课题（卫桂科Z2012273）

摘　　要：目的探讨人类白细胞抗原（HLA）-DR、HLA-G基因多态性与血清总IgE水平的关系。方法将无血缘关系的84例支气管哮喘儿童（哮喘组）和168例无哮喘和特应性疾病的健康儿童（健康对照组）纳入研究。采用Pharmacia UniCAP系统检测哮喘儿童血清总IgE水平，行10种吸人性变应原皮肤试验。应用基因芯片法检测HLA-DR的21个基因位点和基于基质辅助激光解吸电离-飞行时间质谱技术检测HLA-G的9个单核苷酸位点。结果1．哮喘组HLA-DRBI＊070X基因和HLA-DRBI＊llXX基因频率（分别为2．98％和13．69％）高于健康对照组（分别为0．30％和5．95％；X^2=6．915，P〈0．05；X^2=9．478，P〈0．01），优势比（0R）分别为10．570（95％C／：1．215～91．986）和2．790（95％C／：1．429～5．449）；HLA-DRB3（52）＊OIOX基因频率在哮喘组为7．14％，低于健康对照组（13．99％）（X^2=5．854，P〈0．05），OR为0．429（95％CI：0．214～0．862），HLA-DRBI＊15XX在哮喘组的频率为16．67％，显著低于健康对照组（31．70％）（X^2=13．491，P〈0．01），OR为0．431（95％C／：0．263—0．706）。2．H／A—DRBI＊160XX和肌A—DRBI＊3（17）2个等位基因与哮喘儿童血清IgE水平相关（P〈0．05）。其中，HL4-DRBI＊160XX的OR值为0．145，95％C／：0．027—0．781；HLA-DRBI＊3（17）的OR值为1．667，95％C／：1．367～2．033。3．HLA—DRBI＊15XX-HLA-G的rs1704和rs1063320位点的14bp缺失-G单体型（HLA-DRBI＊15XX-14bp缺失-G）携带者患哮喘风险比未携带者降低（wald=13．419，P〈0．001），OR值为0．198，95％CI：0．084—0．471；哮喘组携带该单体型者血清lgE水平[（365．00±943．10）KU／L]也较未携带者[（977．80±14334．90）KU／L]低，差异有统计学意义（F=4．827，P〈0．05）。结论HLA=DRBI＊070X基因和HLA-DRBI＊11XX基因与儿童哮喘易感性相关；而HIA—DRB3（52）＊OIOX基因和HIM—Objective To investigate the association between human leukocyte antigen DR （HLA-DR） gene and HLA-G gene polymorphisms and total IgE levels in children with asthma. Methods Eighty-four unrelated asthmatic individuals and 168 healthy controls without asthma or atopy were involved in the study. All asthmatic children had their serum total IgE levels measured with UniCAP Pharmacia system, and skin-prick test with 10 kinds of inhalant allergens was taken among the asthmatics children. HLA oligonucleotide array was used to detect 21 gene frequencies of HLA-DR and mass ARRAY system （Sequenom） by means of matrix-assisted laser desorption ionisation,while time of flight mass spectrometry method was used for HLA-G gene single nucleotide polymorphism （SNP） genotyping. Results 1. The frequencies of HLA-DRB1 ＊ 070X allele and HLA-DRB1 ＊ 11XX allele among the asthmatics were 2.98% and 13.69%, which were significantly higher than those in healthy controls （0.30% and 5.95 % ,X^2 = 6.915, P 〈 0.05 ;,X^2 = 9. 478, P 〈 0.01 ） , and odds ratios （OR） were 10.570 （95 % C1：1.215 - 91. 986） and 2. 790 （95 % CI： 1. 429 - 5. 449 ） , respectively. HLA-DRB3 （52） ＊ 010X allele was significantly decreased in asthmatic children compared to healthy controls （7.14% vs 13.99 % ,X^2 = 5. 854, P 〈 0.05 ）, and OR were 0. 429 （95 % C1：0.214 - 0. 869）. HLA-DRB1 ＊ 15XX allele were also significantly decreased in asthmatic children compared to healthy controls （ 16.67% vs 31.70% ,X^2 = 13. 491, P 〈 0.01 ）, and OR were 0.431 （ 95% CI： O. 263 - 0. 706 ）. 2. HLA-DRB1 ＊ 160XX, HLA-DRB1 ＊ 3 （17） alleles and the level of total IgE in asthmatic children were significantly correlated （P 〈 0.05）. OR were 0. 145 （95% CI： 0. 027 - 0.781 ） in HLA-DRB1 ＊ 160XX allele and 1. 667 （ 95 % CI： 1. 367 - 2.033 ） in HLA -DRB1 ＊ 3 （17） allele. 3. The individuals with HLA-DRB1 ＊ 15XX-rs1704/rs1063320 locus and 14-bp deletion/G haplotype had lower asthmatic risk than

关 键 词：哮喘 人类白细胞抗原 免疫球蛋白E 基因多态性 儿童 

分 类 号：R725.6[医药卫生—儿科]