平喘固本合剂对支气管哮喘小鼠支气管肺泡灌洗液白细胞介素-17及呼吸道重塑的影响  

Effect of Pingchuanguben Decoction on interleukin-17 of bronchial alveolar lavage fluid and airway inflamma-tion in mice with asthma

作　　者：苗丽燕[1] 韩征利[1] 曲政海[2] 徐雷[2] 

机构地区：[1]临淄区人民医院儿科,山东淄博255400 [2]青岛大学医学院附属医院儿科

出　　处：《中华实用儿科临床杂志》2013年第16期1250-1252,共3页Chinese Journal of Applied Clinical Pediatrics

基　　金：山东省自然科学基金（Y2007C113）;山东省科技发展计划项目（2010Gwz20216）

摘　　要：目的探讨平喘固本合剂对支气管哮喘（哮喘）小鼠支气管肺泡灌洗液（BALF）中IL-17及呼吸道重塑的影响。方法健康雌性昆明系小鼠40只，随机分为4组：正常对照组、哮喘组、平喘固本合剂干预组和布地奈德雾化干预组，每组各10只。采用卵清清蛋白（OVA）建立小鼠哮喘模型，正常对照组腹腔注射PBS，其余3组腹腔注射OVA混悬液，期间平喘固本合剂干预组每日胃饲平喘固本合剂。在激发阶段，正常对照组雾化吸入PBS，其余3组雾化吸入OVA。布地奈德雾化干预组于激发前雾化吸入布地奈德混悬液。采用ELISA法测定BALF中IL-17水平。免疫组织化学法测定肺组织中基质金属蛋白酶-9（MMP-9）、基质金属蛋白酶抑制剂-1（TIMP-1）水平。结果1．平喘固本合剂干预组和布地奈德雾化干预组BALF中IL-17水平明显低于哮喘组（P均〈0．05）；与正常对照组比较，平喘固本合剂干预组BALF中IL-17水平明显升高，差异有统计学意义（P〈0．01）。2．平喘固本合剂干预组和布地奈德雾化干预组呼吸道MMP-9、TIMP-1表达水平和MMP-9／TIMP-1比值明显低于哮喘组，差异均有统计学意义（P均〈0．01）；与正常对照组比较，呼吸道MMP．9表达水平和MMP-9／TIMP-1比值明显增高，差异均有统计学意义（P均〈0．01），2个治疗组间比较，差异均无统计学意义（P均〉0．05）。结论平喘固本合剂对哮喘小鼠具有抑制呼吸道炎症和重塑的作用，在一定程度上可替代布地奈德吸人治疗。Objective To study the effect of Pingchuanguben Decoction on IL-17 level of bronchial alveolar lavage fluid （BALF） and airway remolding in mice with asthma. Methods Forty healthy female Kumning mice were randomly divided into 4 groups （10 rats in each group） ：normal control group, asthma group, Pingehuanguben Decoction group and Budesonide group. The asthmatic models were established by ovalbumin （OVA） ; the normal control group was treated with phosphate buffer solution （PBS liquid） by celiac injection,during this period Pingchuanguben Decoc- tion was administered to the mice in Pingchuanguben Decoction group each day. In stimulation stage, the normal control group was atomization inhalated PBS liquid, and the rest of the 3 groups were given aerosolized OVA instead. Bude- sonide atomization was administered to mice in Budesonide group before stimulation. The levels of IL-17 in BALF were measured with enzyme-linked immunosorbent assay （ ELISA）, and the levels of matrix metalloproteinase-9 （ MMP-9 ） and the tissue inhibitor of metalloproteinase-1 （ TIMP-1 ） in lung tissues were tested with immunohistochemical analysis. Results 1. The levels of IL-17 in BALF in Pingehuanguben Decoction group and Budesonide group were significantly lower than those in asthma group （all P 〈 0.05 ） , and the IL-17 in BALF was increased compared with the normal con- trol group （P 〈 0. O1 ）. 2. The levels MMP-9 ,TIMP-1 and MMP-9/TIMP-1 were decreased in the Pingchuanguben De- coction group and Budesonide group compared with those of asthmatic group （ all P 〈 0.01 ） , and the MMP-9 and MMP-9/TIMP-1 were increased compared with the normal control group（ all P 〈 0.01 ）. There was no significant diffe- rence between Pingchuanguben Decoction group and Budesonide group （ all P 〉 0.05 ）. Conclusions Pingcbuanguben Decoction may restrain airway inflammation and remolding,which can replace Budesonide to a certain extent.

关 键 词：支气管哮喘 白细胞介素-17 基质金属蛋白酶-9 平喘固本合剂 布地奈德混悬液 小鼠 

分 类 号：R285.5[医药卫生—中药学]