伯氏疟原虫感染不同年龄小鼠免疫反应特点  

Characterization of immune response to infection of Plasmodium berghei NK65 in mice of different age

作　　者：单颖[1,2] 刘军[2] 潘艳艳[2] 李莹[2] 曹雅明[2] 

机构地区：[1]辽宁医学院免疫教研室,辽宁锦州121001 [2]中国医科大学免疫教研室

出　　处：《中国公共卫生》2013年第9期1289-1291,共3页Chinese Journal of Public Health

摘　　要：目的了解伯氏疟原虫(Plasmodium berghei NK65,P.b NK65)感染幼年和中年C57BL/6小鼠免疫反应特点。方法 P.b NK65感染3周龄幼年和8月龄中年C57BL/6小鼠,采用流式细胞分析技术检测不同年龄组小鼠脾细胞悬液中髓样树突状细胞(dendritic cells,DCs)数量,表面表达MHCⅡ类分子和CD86分子的DCs数量以及分泌IgG抗体的B细胞数量;ELISA方法检测脾细胞培养上清中IFN-γ和IL-4水平。结果 3周小鼠DCs数量[(40.08±3.54)×105]在感染后5 d明显升高,8月龄鼠在感染后5 d[(103.47±11.07)×105]及8 d[(104.04±1.92)×105]明显升高;3周鼠表面表达MHCⅡ类分子和CD86分子的DCs数量在感染后明显升高,8月龄鼠在感染后8 d表达MHCⅡ类分子[(239.40±16.32)×105]和CD86分子[(148.48±14.84)×105]数量明显高于3周鼠(P<0.05);3周鼠[(251.01±34.02)pg/mL]和8月龄鼠[(584.17±54.03)pg/mL]IFN-γ水平在感染后5 d均明显升高,2组比较差异有统计学意义(P<0.05)。结论 3周和8月龄C57BL/6小鼠感染P.b NK65后,其细胞免疫和体液免疫存在明显差异。Objective To investigate the characteristics of immune response to infection of Plasmodium berghei NK65 （ P. b NK65 ） in young and middle aged C57BL/6 mice. Methods Three weeks and 8 months aged C57BL/6 mice were infected by P. b NK65 parasitized erythrocytes. Flow cytometry was applied to quantitatively analyze myeloid den- dritic cells （DCs） and DCs expressing major histocompatibility complex class II （ MHC II ） or CD86. The levels of interferon gamma（IFN-γ） and interleukin-4 （IL-4）in spleen cell supermatants were dynamically detected by enzyme-linked immunosorbent assay （ELISA）. Results The number of myeloid DCs （40. 08 × 10^5 ）in young aged mice significantly increased 5 days after the infection. The number of myeloid DCs 5 and 8 days after the infection was 103.47 ×10^5 and 104. 04 × 10^5 in the middle aged mice. The numbers of DCs expressing MHCII or CD86 were 50. 16 × 10^5 and 45. 39 × 10^5 in young aged mice,and in middle aged mice the numbers were 239.40 × 10^5 and 148.48 × 10^5, which was markedly more than young aged mice. The level of IFN-γ significantly increased both in young aged（251.01 pg/ml）and middle aged mice（584. 17 pg/ml）. Conclusion The cellular and humoral immune responses to infection of P. b NK65 are dif- ferent between the young and middle aged C57BL/6 mice.

关 键 词：伯氏疟原虫 不同年龄 免疫反应 

分 类 号：R382[医药卫生—医学寄生虫学]