中华眼镜蛇毒口服给药镇痛作用的研究  

Analgesic Effects of Orally Administrating Naja Naja Atra Venom

作　　者：丁晓兰[1] 孙美玲[1] 秦正红[1] 

机构地区：[1]苏州大学药学院,苏州市衰老与神经疾病重点实验室

出　　处：《抗感染药学》2013年第3期180-183,共4页Anti-infection Pharmacy

基　　金：江苏高校优势学科建设工程项目(编号:No.LJ2011139)

摘　　要：目的：研究中华眼镜蛇毒灌服给药对小鼠的镇痛作用。方法：将ICR和昆明种小鼠，采用3种致疼痛模型，即冰醋酸所致扭体反应、小鼠热板法致痛实验和甲醛致炎性疼痛反应；中华眼镜蛇毒经热变性复性处理，将其灌胃给药30-270μg／kg。结果：中华眼镜蛇毒粗毒灌服给药能减少腹腔注射醋酸引起的扭体次数，延长热引起的疼痛反应潜伏期和减少甲醛引起的舔足反应。结论：中华眼镜蛇毒灌服给药具有良好的镇痛作用，且给药途径方便、安全范围大，具有进一步开发成新型镇痛药的潜力。Objective： To study the analgesic effects of orally administrating Naja naja attar venom in mice. Meth- ods： ]CR and Kunming mice were used as three pain models including： acidic acid induced writhing; hot plate test and formalin induced inflammatory pain response.Naja naja atra venom （NNAV） was heat denatured and renatured by cool- ing. NNAV was orally administered at the doses of 30 to 270 μg/kg. Results： NNAV significantly reduced the number of writhing in acidic acid induced writhing test： prolonged the pain latency in hot plate test and reduced the paw licking time in formalin induced pain response. Conclusion： NNAV produced potent analgesic effects. This route of administra- tion is convenient and safe, It has a large therapeutic window, thus guaranteeing the further studies as a potential of nov- el analgesic drug.

关 键 词：中华眼镜蛇毒 镇痛 热板试验 炎性疼痛 扭体 

分 类 号：R965[医药卫生—药理学]