抗纤灵有效单体对活化的肾成纤维细胞株和系膜细胞株的影响  

Effects of utility monomer in Kangxianling to the renal fibroblast cell line and mesangial cell line

作　　者：王东[1] 张江[2] 谢婷婷[1] 吴同茹[1] 李志萃[1] 陈刚[1,3,4] 何立群[1,3,4] 

机构地区：[1]上海中医药大学附属曙光医院,上海200021 [2]合肥师范学院,合肥230601 [3]上海市中医临床重点实验室,上海200021 [4]上海市高校中医内科E-研究院,上海200021

出　　处：《中华中医药杂志》2013年第9期2554-2559,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技重大专项(No.2009ZX09311-003);国家自然科学基金项目(No.81173219);科技部中医药行业科研专项(No.201007005);高等学校博士学科点专项科研基金资助项目(No.20093107110006);上海市科委创新行动计划项目(No.11DZ1973100);上海市教育委员会E-研究院建设计划资助项目(No.E03008);上海高校创新团队建设项目(No.200908)~~

摘　　要：目的:观察抗纤灵有效单体对活化的肾成纤维细胞株和系膜细胞株的影响。方法:采用体外培养肾成纤维细胞株和系膜细胞株的方法,将其分为空白对照组、TGF-β_1 2ng/mL组、TGF-β_12ng/mL+中药10^(-5)g/mL组和TGF-β_12ng/mL+中药10^(-6)g/mL组。采用MTT法观察24h中药单体对两种细胞株的作用以及ELISA法对TGF-β的分泌情况。结果:与空白对照组相比,加入TGF-β_1刺激因子可以促进两种细胞株的活化(P<0.05),以及TGF-β的分泌(P<0.01),中药单体可以抑制活化的细胞株增殖和TGF-β的分泌(P<0.05,P<0.01)。结论:抗纤灵中药单体可以抑制活化的肾成纤维细胞株和系膜细胞株增殖,有效防治肾纤维化,其作用机制可能与其抑制TGF-β的分泌有关。Objective： To observe the effect of utility monomer in Kangxianling to the renal fibroblast cell line and mesangial cell line. Methods： Adopt the method of culturing the renal fibroblast cell line and mesangial cell line in vitro, which were divided into blank control group, TGF-β1 2ng/mL group, TGF-β1 2ng/mL＋Chinese medicine monomer 10-5g/mL group and TGF-β1 2ng/mL ＋Chinese medicine monomer 10 6g/mL group. Observing the effect of Chinese medicine monomer for 24 hours to the two cell lines by MTT assay, and the secretion of TGF-β by ELISA assay. Results： Compared with blank control group, adding TGF-β1-stimulating factor could promote the two cell lines （P〈0.05）, as well as the secretion of TGF-β （P〈0.01）, Chinese medicine monomer could inhibit the activation of two cell lines proliferation and secretion of TGF-β （P〈0.05, P〈0.01）. Conclusion： Utility monomer of Kangxianling can inhibit the activation of the renal fibroblast cell line and mesangial cell line proliferation and secretion of TGF-β, effectively prevent and treat renal fibrosis, and its possible mechanism of action is related to inhibition of TGF-β secretion.

关 键 词：成纤维细胞株 系膜细胞株 体外培养 抗纤灵 中药单体 转化生长因子-Β 

分 类 号：R277.5[医药卫生—中医学]