金欣口服液对呼吸道合胞病毒肺炎幼大鼠白细胞介素-10及肿瘤细胞因子-α的调节作用研究  

Study of Jinxin Oral Liquid on the regulation of IL-10 and TNF-a of RSV infection in young rats

作　　者：赵霞[1] 汪受传[1] 周娣[1] 张沛[1] 徐建亚[1] 

机构地区：[1]南京中医药大学附属医院,南京210029

出　　处：《中华中医药杂志》2013年第9期2604-2606,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.30772822);教育部霍英东基金(No.111042);江苏省"青蓝工程"资助~~

摘　　要：目的:探讨金欣口服液对呼吸道合胞病毒(RSV)肺炎幼大鼠炎症因子白细胞介素-10(IL-10)及肿瘤坏死因子-α(TNF-α)的调节作用。方法:采用细胞培养、实验动物学、ABC-ELISA法等实验技术探讨金欣口服液对机体免疫的调节作用机制。结果:RSV感染大鼠后,促炎症细胞因子TNF-α明显下降(P<0.05),抗炎症细胞因子IL-10的含量增加(P<0.05),TNF-α/IL-10的比值较正常值降低(P<0.05);金欣口服液实验组,TNF-α、IL-10含量均有不同程度的升高,其中以提前给药组增高最为明显,高剂量组次之(P<0.05),TNF-α/IL-10的比值增高,其中以低剂量组最高,高剂量组次之。结论:金欣口服液抗RSV在上调促炎症细胞因子TNF-α含量的同时促进抗炎症细胞因子IL-10的释放,具有调节免疫失衡的作用,从而发挥抗病毒作用。Objective： To study the regulating effect on tumor necrosis factor-α（TNF-α） and interleukin-10（IL-10） of Jinxin oral liquid （JOL） inhibiting respiratory syncytial virus （RSV） infection in young rat. Methods： Cell culture, experimental zoology and ABC-ELISA method were used to detect the regulating effect of JOL on the body immune mechanism. Results： The body pro-inflammatory cytokines TNF-α significantly dropped when viral infection while anti-inflammatory cytokine IL- l0 were increased （P〈0.05）, TNF-α IL-10 ratio was lower than normal （P〈0.05）. After JOL treatment, TNF-α, IL-10 levels were elevated to varying degrees, of which the most obvious was early treatment group, followed by high-dose group （P〈0.05）. TNF-α IL-10 was also increased, and obvious in the low-dose group, following by high-dose group. Conclusion： The mechanism of JOL inhibiting RSV maybe relate to its regulating imbalance of immune by increasing pro-inflammatory cytokines TNF-α and anti- inflammatory cytokine IL-10.

关 键 词：金欣口服液 RSV肺炎 动物实验 细胞因子 白细胞介素-10 肿瘤坏死因子-Α 

分 类 号：R272[医药卫生—中医儿科学]