白地祛脂合剂对人皮脂腺细胞雄激素受体表达的影响  

Effects of Baidi Quzhi Mixture on the expression of human sebaceous gland cells androgen receptor

作　　者：鞠强[1] 李斌[2] 耿琳[2] Christos C Zouboulis 周敏[2] 沈宇弘[2] 陈金鹏[2] 蹇强[2] 宋朋飞[2] 

机构地区：[1]上海交通大学医学院附属仁济医院皮肤科,上海200127 [2]上海中医药大学附属岳阳中西医结合医院,上海200437 [3]Deapartment of Dermatology,Venereology,Allergology and Immunology,Dessau Medical Center,dessau06847

出　　处：《中华中医药杂志》2013年第9期2698-2700,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市卫生局青年基金资助项目(No.2010Y133);上海市卫生局资助"夏氏外科"流派传承研究基地;国家中医药管理局资助"十二五"重点学科--中医皮肤科;国家中医药管理局资助"十二五"重点专科--中西医结合皮肤病专科(皮肤病);上海市科委中药现代化资助(No.11DZ1972004);上海市教委上海高校创新团队建设项目~~

摘　　要：目的:研究白地祛脂合剂对人皮脂腺细胞雄激素受体信使RNA(mRNA)和胞质蛋白表达的影响,探讨中药治疗痤疮的作用机制。方法:采用逆转录-聚合酶链反应(RT-PCR)技术分别检测正常组,低、中、高剂量白地祛脂合剂组作用皮脂腺细胞24h后雄激素受体mRNA的表达情况;采用免疫印迹法(Western Blot)方法检测人皮脂腺细胞胞质蛋白中雄激素受体的表达情况。结果:PCR显示:低、中、高剂量白地祛脂合剂组均使皮脂腺细胞雄激素受体(AR)mRNA表达降低,分别为正常组人皮脂腺细胞的0.592、0.438、0.223倍(P<0.05),呈现一定的量效关系,而高剂量白地祛脂合剂组皮脂腺细胞AR mRNA表达降低最为明显;Western Blot显示:低、中、高剂量白地祛脂合剂组均使皮脂腺细胞雄激素受体胞质蛋白表达降低,分别为正常组人皮脂腺细胞的0.805、0.652、0.550倍(P<0.05),呈现一定的量效关系,而高剂量白地祛脂合剂组皮脂腺细胞AR蛋白降低最为明显。结论:白地祛脂合剂可能通过抑制人皮脂腺细胞AR的表达而具有拮抗雄激素受体对皮脂腺细胞的活性作用,这将为中医药治疗痤疮提供新的治疗途径,有助于从分子层次了解其作用机制。Objective： To study the effect of Baidi Quzhi Mixture on mRNA of human sebaceous gland cells androgen receptor and the expression of cytoplasmic protein, and to explore the mechanism of the treatment of acne by traditional Chinese medicine. Methods： We added different doses of Baidi Quzhi. Mixture in the cultivation of human sebaceous gland cells for 24 hours, and RT-PCR was used to detect the different expressions of androgen receptor mRNA of the normal group, low dose, medium dose and the high dose of Baidi Quzhi Mixture groups. Western Blot was used to detect the expression of androgen receptor in cytoplasmic protein of human sebaceous gland cells. Results： PCR results showed that the low, medium and high doses of Baidi Quzhi Mixture reduced the expression of AR mRNA of the sebaceous cells, it was 0.592, 0.438, 0.223, respectively, and had a dose-effect relationship, the expression of AR mRNA of the high-dose group decreased most significantly （P〈0.05）. Western Blot results： the expression of androgen receptor in cytoplasmic protein of human sebaceous gland cells in all groups decreased,and it was 0.805 ~ 0.652, 0.550, respectively, and the high group most significantly. Conclusion： Baidi Quzhi Mixture may have an antagonistic function on the human sebaceous gland cells activation of androgen receptor through the inhibition of expression of human sebaceous cells AR, which will provide a new therapeutic approach to help to understand the molecular mechanisms of Baidi Quzhi Mixture.

关 键 词：白地祛脂合剂 人皮脂腺细胞 雄激素受体 信使RNA 

分 类 号：R275[医药卫生—中医皮科]