β-细辛醚喷雾给药对哮喘模型豚鼠白细胞介素-4、白细胞介素-16和γ-干扰素水平的影响  

Influence ofβ-asarone on IL-4,IL-16 andγ-IFN level of the asthma model in guinea pig after spray administration

作　　者：李翎[1] 方永奇[1] 邹衍衍[1] 

机构地区：[1]广州中医药大学第一附属医院,广州510405

出　　处：《中华中医药杂志》2013年第9期2704-2706,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广东省科技计划项目(No.2005B33001014)~~

摘　　要：目的:观察哮喘豚鼠经β-细辛醚喷雾给药后,对白细胞介素-4(IL-4)、白细胞介素-16(IL-16)和γ-干扰素(γ-IFN)及小鼠免疫器官指数的影响。方法:将豚鼠分为高、中、低剂量组,阳性药组和模型组,复制哮喘模型,造模7d后,喷雾给药,7d后引喘,记录哮喘发作潜伏期和跌倒潜伏期。取血测定IL-4、IL-16和γ-IFN水平。另取小鼠分为高、中、低剂量组,阳性药组和正常组,喷雾给药7d后,摘取胸腺、脾脏,计算免疫器官指数。结果:β-细辛醚能延长哮喘发作潜伏期和跌倒潜伏期(P<0.01);能降低IL-4的含量、提高IL-16和γ-IFN水平,降低IL-4/γ-IFN比值(P<0.05,P<0.01);能提高小鼠胸腺指数和脾脏指数(P<0.05,P<0.01)。结论:β-细辛醚对哮喘有整体治疗作用;有一定的免疫兴奋作用,并有一定的剂量依赖性,其治疗哮喘可能是通过调节Th1/Th2平衡而实现的。Objective： To observe the influence of 13-asarone on interleukin-4 （IL-4）, interleukin-16 （IL-16） and interferon-y （y-IFN） level of the asthma model in guinea pig after spray administration, and the impact of the drug on the index of immune organs in guinea pig. Methods： Guinea pigs were randomly divided into five groups： high dose group, medium dose group, low dose group, positive control group and model group to make a model of asthma for 7 days, each group were spray administered for another 7 days and then induced asthma. Asthma attack latency and fall down latency were recorded. Blood samples were collected to measure IL-4, IL-16 and y-IFN level. Furthermore, mice were randomly divided into five groups： high dose group, medium dose group, low dose group, positive control group and normal group. Each group were spray administered. After 7 days, the animals were killed. Thymus gland, spleen were weighed to calculate the index of immune organs. Results： 13-asarone extended the asthma attack latency and fall down latency （P〈0.01）, decreased IL-4 level and ratio of IL-4/y-IFN, and increased IL-16 and y-IFN level （P〈0.05, P〈0.01）. It also could enhance thymus index and spleen index （P〈0.05, P〈0.01）. Conclusion： 13-asarone has overall treatment effect on asthma. The drug has certain immune excited effect. The above effects of fi-asarone has a concentration-dependent manner. The therapy effect of 13-asarone in treating asthma may be regulation of the Thl/ Th2 balance.

关 键 词：哮喘 Β-细辛醚 白介素-4 白介素-16 Γ-干扰素 

分 类 号：R285.5[医药卫生—中药学]