干燥综合征模型大鼠心肺功能变化与调节性T细胞的相关性研究  

Correlation analysis between cardiopulmonary function change and Treg in SS rats

作　　者：冯云霞[1] 刘健[2] 万磊[3] 程园园[1] 刘磊[1] 

机构地区：[1]安徽中医学院2010级硕士研究生,合肥230031 [2]安徽中医学院第一附属医院,合肥230031 [3]湖北中医药大学2010级博士研究生,武汉430065

出　　处：《中华中医药杂志》2013年第9期2740-2744,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医药重点学科中医痹病学建设项目(No.国中医药发[2009]30号);安徽省科技厅科研计划项目(No.09020304046)~~

摘　　要：目的:观察干燥综合征(SS)模型大鼠心肺功能变化及探讨与调节性T细胞(Treg)的相关性。方法:将20只SD大鼠随机分为正常对照(NC)组10只和模型(MC)组10只,采用完全弗氏佐剂+同种鼠颌下腺抗原诱导,观察各组大鼠体质量、心肺功能、细胞因子及调节性T细胞变化情况。结果:与NC组比较,MC组大鼠体质量下降,饮水量增加(P<0.01),白介素-17(IL-17)明显升高,白介素-4(IL-4)、Treg表达降低(P<0.05,P<0.01);相关性表明右室舒张末期内径(RV)、左室舒张末期内径(LV)、左心室后壁厚度(LVPW)、左心室短轴缩短率(FS%)、心脏每搏量(SV)、左心房舒张末期内径(LA)、25%肺活量的最大呼气流量(FEF_(25)、50%肺活量的最大呼气流量(FEF_(50))、最大呼气中期流量(MMF)与Treg呈相关性。结论:SS大鼠在发生炎症时,其心肺功能水平呈下降趋势,与Treg表达降低呈相关性,提示Treg调控作用减弱,引起免疫失衡,免疫复合物沉积加重对心肺组织细胞损害,从而导致心肺功能的下降。Objective： To observe the correlation analysis between cardiopulmonary function of SS rats change and CD CD2s Foxp3 Treg. Methods： 20 SD rats were randomly divided into normal control group （NC） and model group （MC）, each group was 10. Complete Freund＇s adjuvant plus same race rat submandibular gland antigen-induced, we observed the rats body weight, heart and lung function, cytokines and regulatory T cells changes. Results： Compared with NC, MC rat body weight decreased, water intake increased （P〈0.01）, IL-17 significantly increased, IL-4 expression of regulatory T cells decreased （P〈0.05, P〈0.01）; correlation indicated that RV, LV, LVPW, FS%, SV, LA, FEF25, FEFs0, MMF and regulatory T cells were correlated. Conclusion： SS rats in inflammation, heart and lung function level downward trend are correlated with the expression of regulatory T cells, that suggesting Treg regulation weakened, causing the immune imbalance, immune complex deposition, thereby increasing the damage on the heart and lung tissue cells, leading to a decline in heart and lung function.

关 键 词：干燥综合征 心肺功能 调节性T细胞 相关性 

分 类 号：R593.2[医药卫生—内科学]